A. Effect of Enzyme Concentration Table 1. Effect of Catalase Concentration on Enzyme Activity noites.Idejand Catalase Time (sec) No dilution 5-fold diluted Questions and Problems Q1. At which enzyme concentration was the reaction the fastest? 11011 At which enzyme concentration was the reaction the slowest? 25-fold diluted

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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**REPORT SHEET | Potato Catalase**

**A. Effect of Enzyme Concentration**

**Table 1. Effect of Catalase Concentration on Enzyme Activity**

| Catalase          | No dilution | 5-fold diluted | 25-fold diluted |
|-------------------|-------------|----------------|-----------------|
| Time (sec)        |             |                |                 |

**Questions and Problems**

**Q1.** At which enzyme concentration was the reaction the fastest?

At which enzyme concentration was the reaction the slowest?

**Q2.** Describe the effect of enzyme concentration on the reaction rate.
Transcribed Image Text:**REPORT SHEET | Potato Catalase** **A. Effect of Enzyme Concentration** **Table 1. Effect of Catalase Concentration on Enzyme Activity** | Catalase | No dilution | 5-fold diluted | 25-fold diluted | |-------------------|-------------|----------------|-----------------| | Time (sec) | | | | **Questions and Problems** **Q1.** At which enzyme concentration was the reaction the fastest? At which enzyme concentration was the reaction the slowest? **Q2.** Describe the effect of enzyme concentration on the reaction rate.
**Section C: Effect of Temperature on Enzyme Activity**

**Table: Effect of Temperature (°C)**

| Temp. (°C)      | Effect on Enzyme Activity        |
|-----------------|----------------------------------|
| Ice             | -                                |
| Room Temperature | -                                |
| 45°C            | -                                |
| Hot Water       | -                                |

**Questions and Problems:**

Q5: At what temperature was the reaction the fastest?

Q6: Describe the effects of temperature on enzyme activity.

---

**Section D: Effect of Protein Denaturation**

**Table: Effect of Protein Denaturation**

| Treatment   | Effect on Enzyme Activity |
|-------------|----------------------------|
| Heated      | -                          |
| Not Heated  | -                          |

**Questions and Problems:**

Q7: Explain the difference in enzyme activity before and after heating.

Q8: Why is a denatured enzyme inactive?

---

**Section B: Effect of pH on Enzyme Activity**

**Table: Effect of pH on Enzyme Activity**

| pH Level       | NaHCO₃  | NaCl    | HCl    |
|----------------|---------|---------|--------|
| 0.01 M         | -       | -       | -      |
| Reaction Rate  | -       | -       | -      | 

**Graph: Reaction Rate vs pH**

(A grid is provided for plotting reaction rate against pH levels)

---

**Questions and Problems:**

Q1: How is catalase activity affected by low pH?

Q2: How is catalase activity affected by high pH?

Q4: What is the optimum pH range for catalase?
Transcribed Image Text:**Section C: Effect of Temperature on Enzyme Activity** **Table: Effect of Temperature (°C)** | Temp. (°C) | Effect on Enzyme Activity | |-----------------|----------------------------------| | Ice | - | | Room Temperature | - | | 45°C | - | | Hot Water | - | **Questions and Problems:** Q5: At what temperature was the reaction the fastest? Q6: Describe the effects of temperature on enzyme activity. --- **Section D: Effect of Protein Denaturation** **Table: Effect of Protein Denaturation** | Treatment | Effect on Enzyme Activity | |-------------|----------------------------| | Heated | - | | Not Heated | - | **Questions and Problems:** Q7: Explain the difference in enzyme activity before and after heating. Q8: Why is a denatured enzyme inactive? --- **Section B: Effect of pH on Enzyme Activity** **Table: Effect of pH on Enzyme Activity** | pH Level | NaHCO₃ | NaCl | HCl | |----------------|---------|---------|--------| | 0.01 M | - | - | - | | Reaction Rate | - | - | - | **Graph: Reaction Rate vs pH** (A grid is provided for plotting reaction rate against pH levels) --- **Questions and Problems:** Q1: How is catalase activity affected by low pH? Q2: How is catalase activity affected by high pH? Q4: What is the optimum pH range for catalase?
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