A. Effect of Enzyme Concentration Table 1. Effect of Catalase Concentration on Enzyme Activity noites.Idejand Catalase Time (sec) No dilution 5-fold diluted Questions and Problems Q1. At which enzyme concentration was the reaction the fastest? 11011 At which enzyme concentration was the reaction the slowest? 25-fold diluted
A. Effect of Enzyme Concentration Table 1. Effect of Catalase Concentration on Enzyme Activity noites.Idejand Catalase Time (sec) No dilution 5-fold diluted Questions and Problems Q1. At which enzyme concentration was the reaction the fastest? 11011 At which enzyme concentration was the reaction the slowest? 25-fold diluted
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
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Does anyone know the answers for any of these?

Transcribed Image Text:**REPORT SHEET | Potato Catalase**
**A. Effect of Enzyme Concentration**
**Table 1. Effect of Catalase Concentration on Enzyme Activity**
| Catalase | No dilution | 5-fold diluted | 25-fold diluted |
|-------------------|-------------|----------------|-----------------|
| Time (sec) | | | |
**Questions and Problems**
**Q1.** At which enzyme concentration was the reaction the fastest?
At which enzyme concentration was the reaction the slowest?
**Q2.** Describe the effect of enzyme concentration on the reaction rate.

Transcribed Image Text:**Section C: Effect of Temperature on Enzyme Activity**
**Table: Effect of Temperature (°C)**
| Temp. (°C) | Effect on Enzyme Activity |
|-----------------|----------------------------------|
| Ice | - |
| Room Temperature | - |
| 45°C | - |
| Hot Water | - |
**Questions and Problems:**
Q5: At what temperature was the reaction the fastest?
Q6: Describe the effects of temperature on enzyme activity.
---
**Section D: Effect of Protein Denaturation**
**Table: Effect of Protein Denaturation**
| Treatment | Effect on Enzyme Activity |
|-------------|----------------------------|
| Heated | - |
| Not Heated | - |
**Questions and Problems:**
Q7: Explain the difference in enzyme activity before and after heating.
Q8: Why is a denatured enzyme inactive?
---
**Section B: Effect of pH on Enzyme Activity**
**Table: Effect of pH on Enzyme Activity**
| pH Level | NaHCO₃ | NaCl | HCl |
|----------------|---------|---------|--------|
| 0.01 M | - | - | - |
| Reaction Rate | - | - | - |
**Graph: Reaction Rate vs pH**
(A grid is provided for plotting reaction rate against pH levels)
---
**Questions and Problems:**
Q1: How is catalase activity affected by low pH?
Q2: How is catalase activity affected by high pH?
Q4: What is the optimum pH range for catalase?
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