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**Substrates of ETC** | **Substrates** | **Stock concentrations** | |-----------------------------------|---------------------------------------------| | Succinate + glutamate (G/S) | 150 mM Glutamate + 150 mM Succinate | | Malate + pyruvate (M/P) | 30 mM Malate + 30 mM Pyruvate | **ATP stock (50 µM):** Make pre-dilutions with 20, 15, 10, 5, 2.5, and 1.25 µM ATP (1000 µL of each). **Calculate the needed volume of the standards!** --- **Inhibitors of ETC** | **Inhibitors** | **Stock concentrations** | **Final concentrations**(in 250 µL) | |------------------|--------------------------------|---------------------------------------| | Rotenone | 400 μg/ml in DMSO | 8 μg/ml | | Oligomycin | 1250 μg/ml in EtOH | 25 μg/ml | | CCCP | 1000 μg/ml in DMSO | 20 μg/ml | | KCN | 400 mM in Tris-buffer | 8 mM | | Antimycin A | 1 mg/ml in DMSO | 20 μg/ml | **Calculate the needed volume!**

**Substrates of ETC** | **Substrates** | **Stock concentrations** | |-----------------------------------|---------------------------------------------| | Succinate + glutamate (G/S) | 150 mM Glutamate + 150 mM Succinate | | Malate + pyruvate (M/P) | 30 mM Malate + 30 mM Pyruvate | **ATP stock (50 µM):** Make pre-dilutions with 20, 15, 10, 5, 2.5, and 1.25 µM ATP (1000 µL of each). **Calculate the needed volume of the standards!** --- **Inhibitors of ETC** | **Inhibitors** | **Stock concentrations** | **Final concentrations**(in 250 µL) | |------------------|--------------------------------|---------------------------------------| | Rotenone | 400 μg/ml in DMSO | 8 μg/ml | | Oligomycin | 1250 μg/ml in EtOH | 25 μg/ml | | CCCP | 1000 μg/ml in DMSO | 20 μg/ml | | KCN | 400 mM in Tris-buffer | 8 mM | | Antimycin A | 1 mg/ml in DMSO | 20 μg/ml | **Calculate the needed volume!**

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Calculate the volume needed!!!

I need to calculate volume for an experiment in laboratory. And I dont know why im confused about the questions. 

I have uploaded the questions, and there is also an apendix they have given us, I dont know if its nedeed to calculate these volumes''???

 

**Substrates of ETC** | **Substrates** | **Stock concentrations** | |-----------------------------------|---------------------------------------------| | Succinate + glutamate (G/S) | 150 mM Glutamate + 150 mM Succinate | | Malate + pyruvate (M/P) | 30 mM Malate + 30 mM Pyruvate | **ATP stock (50 µM):** Make pre-dilutions with 20, 15, 10, 5, 2.5, and 1.25 µM ATP (1000 µL of each). **Calculate the needed volume of the standards!** --- **Inhibitors of ETC** | **Inhibitors** | **Stock concentrations** | **Final concentrations**<br>(in 250 µL) | |------------------|--------------------------------|---------------------------------------| | Rotenone | 400 μg/ml in DMSO | 8 μg/ml | | Oligomycin | 1250 μg/ml in EtOH | 25 μg/ml | | CCCP | 1000 μg/ml in DMSO | 20 μg/ml | | KCN | 400 mM in Tris-buffer | 8 mM | | Antimycin A | 1 mg/ml in DMSO | 20 μg/ml | **Calculate the needed volume!**
Transcribed Image Text:**Substrates of ETC** | **Substrates** | **Stock concentrations** | |-----------------------------------|---------------------------------------------| | Succinate + glutamate (G/S) | 150 mM Glutamate + 150 mM Succinate | | Malate + pyruvate (M/P) | 30 mM Malate + 30 mM Pyruvate | **ATP stock (50 µM):** Make pre-dilutions with 20, 15, 10, 5, 2.5, and 1.25 µM ATP (1000 µL of each). **Calculate the needed volume of the standards!** --- **Inhibitors of ETC** | **Inhibitors** | **Stock concentrations** | **Final concentrations**<br>(in 250 µL) | |------------------|--------------------------------|---------------------------------------| | Rotenone | 400 μg/ml in DMSO | 8 μg/ml | | Oligomycin | 1250 μg/ml in EtOH | 25 μg/ml | | CCCP | 1000 μg/ml in DMSO | 20 μg/ml | | KCN | 400 mM in Tris-buffer | 8 mM | | Antimycin A | 1 mg/ml in DMSO | 20 μg/ml | **Calculate the needed volume!**
Expert Solution
Step 1

For ATP the given values are:

The stock concentration is 50 μM and the final concentrations required are 20, 15, 10, 5, 2.5, 1.25 μM, and the volume that needs to prepare for each one is 1000μl. 

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