The steady-state kinetics of an enzyme are studied in the absence and presence of an inhibitor B (two different concentrations of B were used). The initial rate is given as a function of substrate concentration in the following table: [S] (mmol/L) 1.25 1.67 2.50 5.00 10.00 No inhibitor 1.72 2.06 2.57 3.43 4.11 [(mmol/L)min ¹] 3 mM inhibitor B 1.25 1.55 2.01 2.89 3.70 5 mM inhibitor B 1.00 1.25 1.67 2.52 3.38 What kind of inhibitor is inhibitor B? O mixed O uncompetitive O competitive Submit Part B Determine the apparent Vmax at each inhibitor concentration. Enter your answers separated by commas in requested order. Request Answer without inhibitor, 3 mM, 5 mM Submit Part C Request Answer VΕΙ ΑΣΦ Determine the apparent KM at each inhibitor concentration. Enter your answers separated by commas in requested order. without inhibitor, 3 mM, 5 mM [5] ΑΣΦ p POOL ? ? (mmol/L)min-1 mmol/L
The steady-state kinetics of an enzyme are studied in the absence and presence of an inhibitor B (two different concentrations of B were used). The initial rate is given as a function of substrate concentration in the following table: [S] (mmol/L) 1.25 1.67 2.50 5.00 10.00 No inhibitor 1.72 2.06 2.57 3.43 4.11 [(mmol/L)min ¹] 3 mM inhibitor B 1.25 1.55 2.01 2.89 3.70 5 mM inhibitor B 1.00 1.25 1.67 2.52 3.38 What kind of inhibitor is inhibitor B? O mixed O uncompetitive O competitive Submit Part B Determine the apparent Vmax at each inhibitor concentration. Enter your answers separated by commas in requested order. Request Answer without inhibitor, 3 mM, 5 mM Submit Part C Request Answer VΕΙ ΑΣΦ Determine the apparent KM at each inhibitor concentration. Enter your answers separated by commas in requested order. without inhibitor, 3 mM, 5 mM [5] ΑΣΦ p POOL ? ? (mmol/L)min-1 mmol/L
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
82.21
![The steady-state kinetics of an enzyme are studied in the
absence and presence of an inhibitor B (two different
concentrations of B were used). The initial rate is given as
a function of substrate concentration in the following table:
v[(mmol/L)min-¹]
[S]
No
(mmol/L) inhibitor
1.25
1.67
2.50
5.00
10.00
1.72
2.06
2.57
3.43
4.11
3 mM
inhibitor B
1.25
1.55
2.01
2.89
3.70
5 mM
inhibitor B
1.00
1.25
1.67
2.52
3.38
Part A
What kind of inhibitor is inhibitor B?
mixed
uncompetitive
competitive
Submit
Part B
Request Answer
Determine the apparent Vmax at each inhibitor concentration.
Enter your answers separated by commas in requested order.
without inhibitor, 3 mM, 5 mM
Submit Request Answer
Part C
VE ΑΣΦ
Determine the apparent KM at each inhibitor concentration.
Enter your answers separated by commas in requested order.
VE ΑΣΦ
without inhibitor, 3 mM, 5 mM
?
?
(mmol/L)min-¹
mmol/L](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F52db8ee6-33d1-4aac-bbe4-ebc771de1e49%2F9cbcae96-73b4-44a5-8a05-d6cb9d377b7d%2Fa7h9is_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The steady-state kinetics of an enzyme are studied in the
absence and presence of an inhibitor B (two different
concentrations of B were used). The initial rate is given as
a function of substrate concentration in the following table:
v[(mmol/L)min-¹]
[S]
No
(mmol/L) inhibitor
1.25
1.67
2.50
5.00
10.00
1.72
2.06
2.57
3.43
4.11
3 mM
inhibitor B
1.25
1.55
2.01
2.89
3.70
5 mM
inhibitor B
1.00
1.25
1.67
2.52
3.38
Part A
What kind of inhibitor is inhibitor B?
mixed
uncompetitive
competitive
Submit
Part B
Request Answer
Determine the apparent Vmax at each inhibitor concentration.
Enter your answers separated by commas in requested order.
without inhibitor, 3 mM, 5 mM
Submit Request Answer
Part C
VE ΑΣΦ
Determine the apparent KM at each inhibitor concentration.
Enter your answers separated by commas in requested order.
VE ΑΣΦ
without inhibitor, 3 mM, 5 mM
?
?
(mmol/L)min-¹
mmol/L
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