Bacterial genomes such as that of E. coli typicallyhave only a single origin of replication, from whichreplication proceeds bidirectionally. Pol III, the DNApolymerase responsible for replicating the E. colichromosome, synthesizes DNA at a rate of about1000 nucleotides per second.a. From this information, estimate the minimum generation time of E. coli.b. Under optimal conditions, E. coli have been observedto divide in as little as 17 minutes. Speculate howthis might be possible, given your answer to part (a).
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Bacterial genomes such as that of E. coli typically
have only a single origin of replication, from which
replication proceeds bidirectionally. Pol III, the DNA
polymerase responsible for replicating the E. coli
chromosome, synthesizes DNA at a rate of about
1000
a. From this information, estimate the minimum generation time of E. coli.
b. Under optimal conditions, E. coli have been observed
to divide in as little as 17 minutes. Speculate how
this might be possible, given your answer to part (a).
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