Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if this plasmid was digested with restriction enzyme BamHI.
Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if this plasmid was digested with restriction enzyme BamHI.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if this plasmid was digested with restriction enzyme BamHI.

Transcribed Image Text:You utilised two plasmids in this practical, pOTC and pOTC-A. Plasmids
are often represented using plasmid maps like the one below. This map
shows the positions of recognition sites for a number of restriction
enzymes (ECORV, Pstl, Xbal etc).
Pstl
EcoRV
(6789),
(623)
Хbal
1282)
EcoRI (1538)
BamHI (1545)
Kpnl (1613)
Hindll (1653)
(1691)
Pvull
(6103)
PBCH2.0
Sall
(7693 bp)
Pstl
(2401)
Bstl
Хbal
(4469)
(3188)
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