Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if this plasmid was digested with restriction enzyme BamHI. You utilised two plasmids in this practical, pOTC and pOTC-A. Plasmidsare often represented using plasmid maps like the one below. This mapshows the positions of recognition sites for a number of restrictionenzymes (ECORV, Pstl, Xbal etc).PstlEcoRV(6789),(623)Хbal1282)EcoRI (1538)BamHI (1545)Kpnl (1613)Hindll (1653)(1691)Pvull(6103)PBCH2.0Sall(7693 bp)Pstl(2401)BstlХbal(4469)(3188)

Restriction enzyme are specific DNA cutters and extremely useful while exploring molecular biology…

Answered: Using the plasmid map of pBCH2.0…

Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if this plasmid was digested with restriction enzyme BamHI.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if this plasmid was digested with restriction enzyme BamHI.

You utilised two plasmids in this practical, pOTC and pOTC-A. Plasmids
are often represented using plasmid maps like the one below. This map
shows the positions of recognition sites for a number of restriction
enzymes (ECORV, Pstl, Xbal etc).
Pstl
EcoRV
(6789),
(623)
Хbal
1282)
EcoRI (1538)
BamHI (1545)
Kpnl (1613)
Hindll (1653)
(1691)
Pvull
(6103)
PBCH2.0
Sall
(7693 bp)
Pstl
(2401)
Bstl
Хbal
(4469)
(3188)

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