Enzyme Fragment Size (kb) 1.3, 1.3 Hpall 2.6 HindIII 2.6 ЕcoRI + Hрall 1.3, 0.8, 0.5 ECORI + HindIII 0.6, 0.7, 1.3

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A small DNA molecule was cleaved with several different restriction nucleases, and the size of each fragment was determined by gel electrophoresis.
The following data were obtained.

(a) Is the original molecule linear or circular?
(b) Draw a map of restriction sites (showing distances between sites) that is
consistent with the data given.
(c) How many additional maps are compatible with the data?
(d) What would have to be done to locate the cleavage sites unambiguously
with respect to each other?

Enzyme
Fragment Size (kb)
1.3, 1.3
Hpall
2.6
HindIII
2.6
ЕcoRI + Hрall
1.3, 0.8, 0.5
ECORI + HindIII
0.6, 0.7, 1.3
Transcribed Image Text:Enzyme Fragment Size (kb) 1.3, 1.3 Hpall 2.6 HindIII 2.6 ЕcoRI + Hрall 1.3, 0.8, 0.5 ECORI + HindIII 0.6, 0.7, 1.3
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