A plasmid vector pBS281 is cleaved by the enzymeBamHI (5′ G^GATCC 3′), which recognizes only onesite in the DNA molecule. Human DNA is digestedwith the enzyme MboI (5′ ^GATC 3′), which recognizes many sites in human DNA. These two digestedDNAs are now ligated together. Consider only thosemolecules in which the pBS281 DNA has been joinedwith a fragment of human DNA. Answer the following questions concerning the junction between thetwo different kinds of DNA. a. What proportion of the junctions between pBS281and all possible human DNA fragments can becleaved with MboI?b. What proportion of the junctions between pBS281and all possible human DNA fragments can becleaved with BamHI?c. What proportion of the junctions between pBS281and all possible human DNA fragments can becleaved with XorII (5′ C^GATCG 3′)?d. What proportion of the junctions between pBS281and all possible human DNA fragments can becleaved with BstYI (5′ R^GATCY 3′)? (R and Ystand for purine and pyrimidine, respectively.)e. What proportion of all possible junctions that canbe cleaved with BamHI will result from cases inwhich the cleavage site in human DNA was not aBamHI site in the human chromosome?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
A plasmid vector pBS281 is cleaved by the enzyme
BamHI (5′ G^GATCC 3′), which recognizes only one
site in the DNA molecule. Human DNA is digested
with the enzyme MboI (5′ ^GATC 3′), which recognizes many sites in human DNA. These two digested
DNAs are now ligated together. Consider only thosemolecules in which the pBS281 DNA has been joined
with a fragment of human DNA. Answer the following questions concerning the junction between the
two different kinds of DNA.
a. What proportion of the junctions between pBS281
and all possible human DNA fragments can be
cleaved with MboI?
b. What proportion of the junctions between pBS281
and all possible human DNA fragments can be
cleaved with BamHI?
c. What proportion of the junctions between pBS281
and all possible human DNA fragments can be
cleaved with XorII (5′ C^GATCG 3′)?
d. What proportion of the junctions between pBS281
and all possible human DNA fragments can be
cleaved with BstYI (5′ R^GATCY 3′)? (R and Y
stand for purine and pyrimidine, respectively.)
e. What proportion of all possible junctions that can
be cleaved with BamHI will result from cases in
which the cleavage site in human DNA was not a
BamHI site in the human chromosome?
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