In a process of production of a recombinant protein by E. coli cells, it was observed accumulation of acetate in the culture medium. In this situation, it can be said that:

Q: We transformed E coli cells with a plasmid modified to contain a ‘virulence factor’ which would…

A: A virulance factor is a characteristic or molecule possessed by a microorganisms such as bacteria…

Q: A pyrimidine dimer which is a bulky lesion has mutated an E. coli cell's DNA. Describe both the…

A: The DNA damage can be repaired by several mechanisms that includes direct repair, excision repair,…

Q: Usually, bacteria only make tryptophan when tryptophan is absent or available in low concentration.…

A: Normally, bacteria regulate the synthesis of tryptophan through the trp operon. When tryptophan…

Q: Explain Avery-MacLeod-McCarty experiment (the 1940's) with capsule forming bacteria (smooth) and…

A: Bacteria are single-celled microorganisms found in all environments on Earth, including soil, water,…

Q: The Borrelia hermsii spirochete causes a relapsing fever, characterized by multiple periods of high…

A: Borrelia hermsii are spirochete bacteria that have a thin long and spiral shape and move with the…

Q: Select the true statements: A.) It is possible, through genetic engineering, to fuse a fluorescent…

A: Explanation:     -A fluorescent fusion protein can indeed be produced by fusing a fluorescent…

Q: Provide a detailed description and hand drawn figure of each of the following. (1) Mismatch…

A: Although the genetic variation is important for evolution, the survival of the individual demands…

Q: You generate several temperature sensitive mutant strains of bacteria. To study what genes might be…

A: Introduction :- Bacterial mutants frequently lose a growth characteristic (such as the inability to…

Q: The soil bacterium Agrobacterium tumefaciens, also regarded as 'nature's own genetic engineer', is…

A: As per our guidelines, we are supposed to answer only three sub-parts. Kindly repost the question…

Q: Emtricitabine (2,3-dideoxy-5-fluoro-3'-thiacytidine, abbreviated as FTC) is a nucleoside analog that…

A: HIV: (human immunodeficiency virus) this is a virus that can attack our immune system. This virus…

Q: ) O The bacteriophage can exit the lysogenic state depending on the condition of host c 3) O While…

A: Bacteriophage is a very very small virus that lives on the bacteria. it has two types of cycles the…

Q: I read that vinyl chloride exposure is associated with an increased risk of a rare form of liver…

A: Cancer is the condition of uncontrolled cell division.

Q: How can you tell if you have had a successful induced transformation using: "C" = control, "T"…

A: Transformation refers to the process of introducing foreign DNA (such as plasmid DNA) into bacterial…

Q: If conjugation has occurred between the provided E. coli strains I and II, what might we expect to…

A: Introduction  Recombinant refers to something that has been produced by the combination of genetic…

Q: You have two E. coli cultures. One culture is a wild-type culture, and the other contains the lacOc…

A: Escherichia coli (E. coli) is a type of bacteria that is commonly found in the lower intestine of…

Q: can you explain why we are hoping to obtain white colonies? (Please explain both why blue colonies…

A: In recombinant DNA technology vector is any DNA molecule either from plant, virus, plasmid or…

Q: Direct repair of pyrimidine dimer formation in E. Coli can be accomplished by nucleotide excision.…

A: Escherichia coli, commonly abbreviated as E. coli, is a gram-negative, rod-shaped bacterium that is…

Q: In the Hershey–Chase experiment, the radioactive label 32P was present inside bacterial cells (i.e.,…

A: DNA is an organic molecule that transmits information from generation to generation. Several…

Q: Two auxotrophic triple Escherichia coli strains (A: met- phe- ade- val+ bio+ thr+ and B: met+ phe+…

A: Auxotropic strains are the strains of microorganisms that have undergone mutations and as a result…

Q: The table above shows the predicted results. A plus means when the strain with a mutation in the…

A: The method described in question is one usually used by biochemists and enzymologists to decipher…

Q: Choose two genes from Figure 4.6 and draw a graph to represent the change in transcription over…

A: Transcription:  All the necessary information required to make proteins is stored n the nucleotide…

Q: Cytopathic effects (CPE) are visible changes in cells due to: A)viral infection B)mutations…

A: Cytopathic effects are structural changes in a cell which can be easily viewed under a compound…

Q: In the Hershey Chase experiment, why was the localization of radio-labeled phage of macromolecules…

A: Macromolecules are large molecules composed of repeating subunits or monomers which are typically…

Q: Suppose you want to figure out what genes are involved in the production of proline (Pro) in a…

A: Introduction :- Proline is one of the  essential amino acid .It is the only proteinogenic secondary…

Q: When Griffith injected mice with a combination of live rough-strain and heat-killed smooth-strain…

A: Frederick Griffith was responsible for conducting an experiment using Streptococcus pneumoniae,…

Q: In E. coli, the gene bioD+ encodes an enzyme involved in biotin synthesis, and galK+ encodes an…

A: Gene transfer is the process of insertion of unrelated DNA into cells. There are three mechanisms of…