Select the true statements: A.) It is possible, through genetic engineering, to fuse a fluorescent molecule (example GFP) with a non-fluorescent protein to create a fluorescent fusion protein. B.) Proteins are created during gene transcription. C.) It is possible to observe a fluorescent protein immediately after the plasmid coding for this protein is introduced into a cell. D.) The plasma coding for a fluorescent protein is also fluorescent. E.) It is possible to obtain a different color fluorescent fusion protein by replacing the sequence coding for the fluorescent protein in the plasmid for a sequence coding for a fluorescent protein by a different color sequence.
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
Select the true statements:
A.) It is possible, through genetic engineering, to fuse a fluorescent molecule (example GFP) with a non-fluorescent protein to create a fluorescent fusion protein.
B.) Proteins are created during gene transcription.
C.) It is possible to observe a fluorescent protein immediately after the plasmid coding for this protein is introduced into a cell.
D.) The plasma coding for a fluorescent protein is also fluorescent.
E.) It is possible to obtain a different color fluorescent fusion protein by replacing the sequence coding for the fluorescent protein in the plasmid for a sequence coding for a fluorescent protein by a different color sequence.
Explanation:
-A fluorescent fusion protein can indeed be produced by fusing a fluorescent molecule with a non-fluorescent protein. Through a procedure known as genetic engineering, this is possible. The process of altering an organism's traits by modifying its genes is known as genetic engineering. In this instance, fluorescent proteins are created by manipulating their genes.
- A fluorescent protein's plasma coding is not necessarily fluorescent. However, a fluorescent protein can be seen right away after the plasmid that codes for it are inserted into a cell. This is possible because the plasmid can splice the fluorescent protein coding into the cell's genome.
- By swapping out the sequence in the plasmid that codes for the fluorescent protein with one that codes for a fluorescent protein of a different hue, it is also feasible to produce a fluorescent fusion protein of a different color. To accomplish this, use a separate plasmid that encodes for a different fluorescent protein color.
- During the transcription of genes, proteins are not produced. The process of copying a gene from DNA to RNA is called transcription. Proteins are then produced using RNA.
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