a) b) Consider the following proteins and their properties. Molar Mass (kDa) Description Protein pl A 6.1 22.5 B 4.5 71.6 C 8.4 65.8 BCD 7.2 48.0 monomer homodimer single peptide chain Order the proteins (eluted first to last) on an anion exchange column at pH 6.5. aß dimer (a chain is twice the size of the B chain) Protein Elutes 1st Elutes 2nd Elutes 3rd Elutes last look like if prior to loading each protein in a separate lane, you boiled each protein sample in If you performed SDS-polyacrylamide gel electrophoresis, what would the gel sample buffer containing ẞ-ME and SDS? Molecular weight markers A B C D KDA 97,400 66,200 45,000 31,000 21,500 14,400

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Chapter3: Biological Macromolecules
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Problem 23CTQ: Amino acids have the generic structure seen below, where R represents different carbon-based side...
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a)
b)
Consider the following proteins and their properties.
Molar Mass (kDa) Description
Protein pl
A
6.1
22.5
B
4.5 71.6
C
8.4 65.8
BCD
7.2 48.0
monomer
homodimer
single peptide chain
Order the proteins (eluted first to last) on an anion exchange column at pH 6.5.
aß dimer (a chain is twice the size of the B chain)
Protein
Elutes 1st
Elutes 2nd
Elutes 3rd
Elutes last
look like if prior to loading each protein in a separate lane, you boiled each protein sample in
If you performed SDS-polyacrylamide gel electrophoresis, what would the gel
sample buffer containing ẞ-ME and SDS?
Molecular weight markers
A
B
C
D
KDA
97,400
66,200
45,000
31,000
21,500
14,400
Transcribed Image Text:a) b) Consider the following proteins and their properties. Molar Mass (kDa) Description Protein pl A 6.1 22.5 B 4.5 71.6 C 8.4 65.8 BCD 7.2 48.0 monomer homodimer single peptide chain Order the proteins (eluted first to last) on an anion exchange column at pH 6.5. aß dimer (a chain is twice the size of the B chain) Protein Elutes 1st Elutes 2nd Elutes 3rd Elutes last look like if prior to loading each protein in a separate lane, you boiled each protein sample in If you performed SDS-polyacrylamide gel electrophoresis, what would the gel sample buffer containing ẞ-ME and SDS? Molecular weight markers A B C D KDA 97,400 66,200 45,000 31,000 21,500 14,400
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