1. Draw the appropriate titration curve for the tripeptide Met-Lys-Val starting at pH 1 and ending at pH 12. Label the pKas and the pI. Draw the two different forms of the molecule that is present at each buffering region. 2. Draw the structure of the peptide Arg-Met-His-Val-Glu and circle the coplanar atoms in one peptide bond. 3. Determine the pI for the peptide given in question 2 above.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
1. Draw the appropriate titration curve for the tripeptide Met-Lys-Val starting at pH 1 and ending at pH 12. Label the pKas and the pI. Draw the two different forms of the molecule that is present at each buffering region.
2. Draw the structure of the peptide Arg-Met-His-Val-Glu and circle the coplanar atoms in one peptide bond.
3. Determine the pI for the peptide given in question 2 above.
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