25. The protease papain, which can be isolated from papaya fruit, is a monomer with one active site per protein molecule. The activity of purified papain enzyme was measured at various substrate concentrations and plotted as initial velocity on the following graph. (mM/min) 35 30 25 5/5° 20 20 15 10 5 25 25 20 0 -10 -505 10 15 20 1 (mM) 1 [Substrate] 30 30 Considering that the enzyme concentration was held constant in these experiments at 1.2 × 107 M, answer the following questions about this enzyme. a. Calculate Km for this substrate. b. Calculate Vmax for this substrate. c. Calculate keat for papain with this substrate. d. What fraction of total enzyme has substrate bound when the substrate concentration is 2.5 × 10 4 M?
25. The protease papain, which can be isolated from papaya fruit, is a monomer with one active site per protein molecule. The activity of purified papain enzyme was measured at various substrate concentrations and plotted as initial velocity on the following graph. (mM/min) 35 30 25 5/5° 20 20 15 10 5 25 25 20 0 -10 -505 10 15 20 1 (mM) 1 [Substrate] 30 30 Considering that the enzyme concentration was held constant in these experiments at 1.2 × 107 M, answer the following questions about this enzyme. a. Calculate Km for this substrate. b. Calculate Vmax for this substrate. c. Calculate keat for papain with this substrate. d. What fraction of total enzyme has substrate bound when the substrate concentration is 2.5 × 10 4 M?
Chapter21: Heparin Infusion Calculations
Section: Chapter Questions
Problem 1.4P
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![25. The protease papain, which can be isolated from papaya fruit, is
a monomer with one active site per protein molecule. The
activity of purified papain enzyme was measured at various
substrate concentrations and plotted as initial velocity on the
following graph.
(mM/min)
35
30
25
5/5°
20
20
15
10
5
25
25
20
0
-10 -505 10
15
20
1
(mM) 1
[Substrate]
30
30](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa5644016-779f-4740-bb26-bd379069d798%2Fe9c61de6-8510-4d91-943a-158c70afa6c2%2Fela0ken_processed.png&w=3840&q=75)
Transcribed Image Text:25. The protease papain, which can be isolated from papaya fruit, is
a monomer with one active site per protein molecule. The
activity of purified papain enzyme was measured at various
substrate concentrations and plotted as initial velocity on the
following graph.
(mM/min)
35
30
25
5/5°
20
20
15
10
5
25
25
20
0
-10 -505 10
15
20
1
(mM) 1
[Substrate]
30
30
Transcribed Image Text:Considering that the enzyme concentration was held constant in
these experiments at 1.2 × 107 M, answer the following
questions about this enzyme.
a. Calculate Km for this substrate.
b. Calculate Vmax for this substrate.
c. Calculate keat for papain with this substrate.
d. What fraction of total enzyme has substrate bound when the
substrate concentration is 2.5 × 10 4 M?
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