2. Use the table to answer the following questions about the peptide you drew in question 1. Amino PK1 pk2 PKR Acid (-COOH) (-NH3+) (R-group) Lys 2.18 8.95 10.53 Thr 2.11 9.62 Gln 2.17 9.13 His 1.82 9.17 6.00 Val 2.32 9.62 a. Determine the net charge of the peptide at pH 0 and 14? b. Estimate the pl for this peptide. Is this an acidic or basic peptide?

Please help me solve this I found that the pH of 0 to be +3 at pH 0 and -1 at pH 14. Need to confirm if this is true. I used the n terminus, lysine r group and histidine r group only to solve in question 1 I drew the KTQHV peptide with k being lysine, t threonine, g glutamine, h histidine and v being valine. I drew one ionizable group as well with it being histidine and indicated the approximate charge in question 1.