2. Use the table to answer the following questions about the peptide you drew in question 1. Amino PK1 pk2 PKR Acid (-COOH) (-NH3+) (R-group) Lys 2.18 8.95 10.53 Thr 2.11 9.62 Gln 2.17 9.13 His 1.82 9.17 6.00 Val 2.32 9.62 a. Determine the net charge of the peptide at pH 0 and 14? b. Estimate the pl for this peptide. Is this an acidic or basic peptide?
2. Use the table to answer the following questions about the peptide you drew in question 1. Amino PK1 pk2 PKR Acid (-COOH) (-NH3+) (R-group) Lys 2.18 8.95 10.53 Thr 2.11 9.62 Gln 2.17 9.13 His 1.82 9.17 6.00 Val 2.32 9.62 a. Determine the net charge of the peptide at pH 0 and 14? b. Estimate the pl for this peptide. Is this an acidic or basic peptide?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Question
Please help me solve this I found that the pH of 0 to be +3 at pH 0 and -1 at pH 14. Need to confirm if this is true. I used the n terminus, lysine r group and histidine r group only to solve in question 1 I drew the KTQHV peptide with k being lysine, t threonine, g glutamine, h histidine and v being valine. I drew one ionizable group as well with it being histidine and indicated the approximate charge in question 1.
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