2) The following experimental data were collected while studying an intestinal peptidase that degrades the dipeptide glycylglycine into individual glycine amino acids: Product formed Glycylglycine + H₂O → 2 glycine [S] (MM) (μM/min) 1.5 0.21 2.0 0.24 3.0 0.28 4.0 0.33 8.0 0.40 16.0 0.45 Use graphical analysis to determine Vmax and Km for this preparation of enzyme and substrate.
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Please help and explain 2)
![2) The following experimental data were collected while studying an intestinal peptidase that degrades the
dipeptide glycylglycine into individual glycine amino acids:
Product formed
Glycylglycine + H₂O → 2 glycine
[S] (MM)
(μM/min)
1.5
0.21
2.0
0.24
3.0
0.28
4.0
0.33
8.0
0.40
16.0
0.45
Use graphical analysis to determine Vmax and Km for this preparation of enzyme and substrate.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe0719576-6288-4966-bcac-ea0ff9b5df4d%2Fdd51a202-07b0-430c-9bc5-630fdf6d4445%2Fimjol3i_processed.jpeg&w=3840&q=75)
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- For production of penicillin (C16H18O4N2S) using Penicillium mold, glucose (C6H12O6) is used as a substrate and phenylacetic acid (C3H3O2) is added as a precursor. The stoichiometry for overall synthesis is: 1.67 C6H1206 + 2NH3 + 0.502+ H2SO4+ C8H3O2 → C16H1804N2S + 2CO2+ 9H2O a) What is the maximum theoretical yield of penicillin from glucose in g/g? b) When results from a particular penicillin fermentation were analyzed, it was found that 24% of the glucose had been used for growth, 70% for cell maintenance activities (such as membrane transport and macromolecule turnover) and only 6% for penicillin synthesis. Calculate the yield of penicillin from glucose in g/g under these conditions. c) Batch fermentation under the conditions described in part b) is carried out in a 100-liter tank. Remember that only 6% of the glucose is used for penicillin synthesis. Initially, the tank is filled with nutrient medium containing 50 g L' glucose and 4 g L phenylacetic acid. If the reaction is…Following is a Dixon plot for PNPP inhibition by inorganic phosphates. What answer choice shows the correct combination of the mode of inhibition and Ki of the EI complex?Draw the Mchelis Menton plot and the Line Weaver Burk Plot using the data given below and find the Vmax and Km from the Plot.
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- The parietal cells of the stomach lining contain membrane “pumps” that transport hydrogen ions from the cytosol (pH 7.0) into the stomach,contributing to the acidity of gastric juice (pH 1.0). Calculate the free energy required to transport 1 mol of hydrogen ions through these pumps. Assume a temperature of 37 °C.Explain the molecular basis behind the reported observation in the parameter measured below:Proline racemase catalyzes the conversion between L-proline and D-proline. The Km and kcat for this reaction are 0.15 M and 550/sec respectively. If the enzyme concentration is 1.45 X 10-5 mmole/ml what is the Vmax of this reaction?