NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 8, Problem 5MC
You are trying to isolate a mutant of wild-type E. coli that requires histidine for growth. This can best be done using
1)direct selection.
2)replica plating.
3)penicillin enrichment.
4)the Ames test.
5)reversion.
a) 1, 2
b) 2, 3
c) 3, 4
d) 4, 5
e) 1, 5
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
1) Which technique is best suited to determining which genes are activated in a bacterium during infection while causing disease in a person.
a) SDS-page
b) microarray analysis
c) RFLP analysis
d) clone library analysis
2)Which of the following is not an application of PCR?
a) Determine if two people are related.
b) Identify a bacterial pathogen in a patient sample.
c) Determine the gene sequence of the gene that codes for a bacterial enterotoxin.
d) These are all applications of PCR.
You followed all the steps correctly with a kit you bought last week. However, NO purified DNA came out of the miniprep (More than one answer possible).
a) The plasmid was lost during culture growth
b) Your tears from doing the same experiment again and again contaminated the reagents, causing them not to work
c) Depurination of the DNA led to loss of genetic material
d) Incomplete lysis of the cells after adding lysis buffer
Consider the following experiment. First, large populations of two mutant strains of Escherichia coli are mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45 colonies grew. Which of the following may explain this result?
A) The colonies may be due to back mutation (reversion).
B) The colonies may be due to recombination.
C) Either A or B is possible.
D) Neither A nor B is possible.
Chapter 8 Solutions
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
Ch. 8 - Prob. 1SACh. 8 - Why is deleting one nucleotide generally more...Ch. 8 -
3. What type of mutation in an operon is most...Ch. 8 -
4. What is meant by "proofreading" with respect...Ch. 8 - Why would a cell use SOS repair, considering that...Ch. 8 - Why is replica plating used to isolate an...Ch. 8 - What is transduction?Ch. 8 -
8. How is an F+ strain different from an Hfr...Ch. 8 - Name four mobile genetic elements.Ch. 8 - Why are R plasmids important?
Ch. 8 - UV light exposure forms a) covalent bonds between...Ch. 8 -
2. If cells were exposed to UV light, the highest...Ch. 8 -
3. Penicillin enrichment of mutants works on the...Ch. 8 -
4. Mechanisms that repair errors in nucleotide...Ch. 8 -
5. You are trying to isolate a mutant of...Ch. 8 -
6. All plasmids
1) carry genes for antimicrobial...Ch. 8 -
7. Adding DNase to a mixture of donor and...Ch. 8 -
8. An F pilus or its equivalent is essential...Ch. 8 - A plasmid that can replicate in E. coli and...Ch. 8 -
10. The frequency of transfer of an F' molecule...Ch. 8 -
1. Some bacteria may have higher mutation rates...Ch. 8 -
2. A pharmaceutical researcher is disturbed to...Ch. 8 - Prob. 1CTCh. 8 -
2. You have isolated a strain of E. coli that is...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Sequence of which of the following cannot be determined using the Maxam Gilbert method?a) Bacteriab) Plantsc) Bacteriophage T7d) Plasmidarrow_forwardWe have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media: A) Donor and recipient strain mixed together (control - no treatment). B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells). The results: Tubes A, B, and D: HGT was observed. Tube C: HGT was NOT observed. Based on this, which type of HGT was occurring? Conjugation,…arrow_forwardDescribe how would you determine the following using a real-time PCR instrument. A) The quantity and quality of a DNA sample. B) Whether an individual had cystic fibrosis from a genomic DNA sample. C) If several bacterial pathogens were present in DNA extracted from a food sample.arrow_forward
- The percentage of the agarose gel is important because a) it influences the rate of migration of the fragments b) it may cause some DNA molecules to replicate c) some DNA nucleotides may degraded due to chemical reactions with the gel d) some DNA molecules may sink to the bottom of the well and not migrate What is the purpose of a buffer used in electrophoresis? a) Increase/decrease the current b) Conduct the current and protect the samples c) Disrupt the bands d) Stop the band migration Which of the following is an additional use of the gel electrophoresis technique? a) To express DNA b) To separate RNA c) To amplify RNA d) To amplify genesarrow_forwardWhich of the following are true regarding assay attributes? List all that are true. a) Accuracy represents the closeness of the assay result to the “true value”. b) Robustness represents the ability of a method to distinguish between the analyte and similar components. c) Precision is determined by replicate analysis of a reference standard or well-characterized material. d) A robust assay would have a very narrow range of acceptable assay conditions. For those that were not true in question 1, correct the statement(s).arrow_forward17) What gives Aspergillus sydowii colonies their hairy appearance? a) Hyphae () b) Stipes c) Growing media ) d) Ascocarpoda Jack has designed one primer which is complementary to the DNA of E. coli. He then used that primer for a PCR reaction using DNA isolated from a mixture of bacteria. He got no PC reaction product and hence concluded that there is absolutely no E. coli in the bacteria mixture. His conclusion is .... () True ) Falsearrow_forward
- Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.arrow_forwardSolution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual). Which effect does NaOH have on E. coli DNA A) It denatures genomic DNA and plasmid DNA. B) It denatures genomic DNA and but not plasmid DNA. C) It denatures plasmid DNA but not genomic DNA. D) It denatures neither genomic DNA nor plasmid DNA. E) This is unpredictable.arrow_forwardWhich is used for cloning eukaryotic genes but not prokaryotic genes?a) Restriction enzymesb) DNA ligasec) Reverse transcriptased) Vectore) Selectable markerarrow_forward
- All of the following apply to Luria and Delbruck’s 1943 study of mutation rates in E. Coli and T1 phage except: A) it served as an example of an inflexible test B) it involved looking for T1-resistant bacteria as an end measure C) it showed that numbers of mutant organisms observed after T 1 phage were added to the culture tended to vary from experiment to experiment D) it rejected the possibility of adaptive mutations E) it supported the concept of spontaneous mutation ratesarrow_forwardWhen cloning a piece of DNA, the purpose of using the LacZ blue-white colony method is to A) Remove bacteria that do not have recombinant vectors B) Remove bacteria that do not have the DNA insert of interest Remove linear DNA Distinguish colonies that have recombinant vectors from those with non-recombinant vectors. E) Remove bacteria that have not taken up the vectorarrow_forwardWhich of the following does not act as a restriction enzyme?a) EcorIb) BamHIc) HindIIId) polydeoxyribonucleotide synthasearrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license