NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
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Chapter 8, Problem 2CT
You have isolated a strain of E. coli that is resistant to penicillin, streptomycin, chloramphenicol, and tetracycline. You also observe that when you mix this strain with cells of E. coli that are sensitive to the four antibiotics, they become resistant to streptomycin, penicillin, and chloramphenicol but remain sensitive to tetracycline. Explain what is going on.
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You are designing a phage therapy for a cystic fibrosis patient with an multi-antibiotic resistant Mycobacterium infection. The Mycobacterium infection is preventing the patient from taking immunosuppressant drugs that are needed for a successful lung transplant. The idea is that introducing the right phage to the patient will kill the Mycobacterium cells and allow the patient to go on immunosuppressant drugs that will ensure the new lung is not rejected by the body.
You need to find a phage that infects the Mycobacterium you isolated from the patient.
Which TWO options have the highest chance of success?
A. Screening a colleague's library of known Mycobacterium phages for phage that infect the patient's Mycobacterium isolate
b. Choosing likely candidates from analysis of phage 16S rRNA phylogeny in the tree of life
C. Isolating new phage from soil using a plaque assay with the Mycobacterium isolate
D. Obtaining a very well-studied phage, such as T4 bacteriophage, that…
You are designing a phage therapy for a cystic fibrosis patient with an multi-antibiotic resistant Mycobacterium infection. The Mycobacterium infection is preventing the patient from taking immunosuppressant drugs that are needed for a successful lung transplant. The idea is that introducing the right phage to the patient will kill the Mycobacterium cells and allow the patient to go on immunosuppressant drugs that will ensure the new lung is not rejected by the body.
You finally find a phage that you think will work because it attaches to and injects its genome into the patient's Mycobacterium isolate. Next, you need to be sure of what characteristics of the phage infection cycle? Pick all that are true
A. That the phage delivers new antibiotic resistance genes to Mycobacterium via transduction
B. That the correct proteins are expressed during the phages metabolism
C. That the infection is lytic
D.That lysogeny is the primary infection pathway
Chapter 8 Solutions
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
Ch. 8 - Prob. 1SACh. 8 - Why is deleting one nucleotide generally more...Ch. 8 -
3. What type of mutation in an operon is most...Ch. 8 -
4. What is meant by "proofreading" with respect...Ch. 8 - Why would a cell use SOS repair, considering that...Ch. 8 - Why is replica plating used to isolate an...Ch. 8 - What is transduction?Ch. 8 -
8. How is an F+ strain different from an Hfr...Ch. 8 - Name four mobile genetic elements.Ch. 8 - Why are R plasmids important?
Ch. 8 - UV light exposure forms a) covalent bonds between...Ch. 8 -
2. If cells were exposed to UV light, the highest...Ch. 8 -
3. Penicillin enrichment of mutants works on the...Ch. 8 -
4. Mechanisms that repair errors in nucleotide...Ch. 8 -
5. You are trying to isolate a mutant of...Ch. 8 -
6. All plasmids
1) carry genes for antimicrobial...Ch. 8 -
7. Adding DNase to a mixture of donor and...Ch. 8 -
8. An F pilus or its equivalent is essential...Ch. 8 - A plasmid that can replicate in E. coli and...Ch. 8 -
10. The frequency of transfer of an F' molecule...Ch. 8 -
1. Some bacteria may have higher mutation rates...Ch. 8 -
2. A pharmaceutical researcher is disturbed to...Ch. 8 - Prob. 1CTCh. 8 -
2. You have isolated a strain of E. coli that is...
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