NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 8, Problem 6SA
Why is replica plating used to isolate an auxotrophic mutant from a prototrophic parent?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Describe how replica plating is used to detect and isolate auxotrophic mutants.
What is micropropagation? Describe the different in-vitro stages in themicropropagation of any named crop.
How is an auxotroph different from a prototroph?
Chapter 8 Solutions
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
Ch. 8 - Prob. 1SACh. 8 - Why is deleting one nucleotide generally more...Ch. 8 -
3. What type of mutation in an operon is most...Ch. 8 -
4. What is meant by "proofreading" with respect...Ch. 8 - Why would a cell use SOS repair, considering that...Ch. 8 - Why is replica plating used to isolate an...Ch. 8 - What is transduction?Ch. 8 -
8. How is an F+ strain different from an Hfr...Ch. 8 - Name four mobile genetic elements.Ch. 8 - Why are R plasmids important?
Ch. 8 - UV light exposure forms a) covalent bonds between...Ch. 8 -
2. If cells were exposed to UV light, the highest...Ch. 8 -
3. Penicillin enrichment of mutants works on the...Ch. 8 -
4. Mechanisms that repair errors in nucleotide...Ch. 8 -
5. You are trying to isolate a mutant of...Ch. 8 -
6. All plasmids
1) carry genes for antimicrobial...Ch. 8 -
7. Adding DNase to a mixture of donor and...Ch. 8 -
8. An F pilus or its equivalent is essential...Ch. 8 - A plasmid that can replicate in E. coli and...Ch. 8 -
10. The frequency of transfer of an F' molecule...Ch. 8 -
1. Some bacteria may have higher mutation rates...Ch. 8 -
2. A pharmaceutical researcher is disturbed to...Ch. 8 - Prob. 1CTCh. 8 -
2. You have isolated a strain of E. coli that is...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What is the difference between complete medium and minimal medium? How are complete media and minimal media to which one or more nutrients have been added (selective media) used to isolate auxotrophic mutants of bacteria?arrow_forwardDescribe direct selection and indirect selection of identifying mutants. What are auxotroph and prototroph?arrow_forwardIs the genus alcaligenes gelatin negative or postive?arrow_forward
- Briefly explain how replica plating can identify new bacterial auxotrophs.arrow_forwardWhat is Auxotroph and Prototroph?arrow_forwardYou co-culture the following bacterial strains: an Hfr prototroph and an F- auxotroph for the genes mal, met, mtl, and xyl. You interrupt conjugation at various time points and place the mixtures on media plates lacking each of the nutrients. Based on the results shown on the right, what is the order of these four genes along the bacterial chromosome? Explain your reasoning.arrow_forward
- You co-culture the following bacterial strains: an Hfr prototroph and an F- auxotroph for the genes mal, met, mtl, and xyl. You interrupt conjugation at various time points and place the mixtures on media plates lacking each of the nutrients. Based on the results shown in the image, what is the order of these four genes along the bacterial chromosome? Please explain!arrow_forwardWhy is Agrobacterium mediated genetic transformation described as Natural Genetic engineering in plants?arrow_forwardWhich of the following is used as a marker in YAC vectors? A) centromere B) X Gal C) auxotrophic markers D) ARSarrow_forward
- With regard to studying the mechanism of conjugation,what is the purpose of using a U-tube?arrow_forwardWhy is the use of auxotrophy not a good method for controllingthe growth of a genetically modified organism?arrow_forwardYou are using a T7 promotor in yeast, your protein is a large complex protein. Which would be the ideal vector to use to manufacture this in yeast and which one would you use in E. coli?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage Learning
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license