NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
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Textbook Question
Chapter 8, Problem 8MC
An F pilus or its equivalent is essential for
1)DNA-mediated transformation.
2)chromosome transfer by conjugation.
3)plasmid transfer by conjugation.
4)generalized transduction.
5)cell movement.
a) 1, 2
b) 2, 3
c) 3, 4
d) 4, 5
e) 1, 5
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Which of the statements is TRUE about conjugation?
a)
Plasmid DNA is transferred from a donor (F+) cell
b)
It may only be performed by competent cells
c)
DNA is always integrated into recipient chromosome
d)
It may be specialized or generalized
Which process listed below allows genetic material to be transferred from a virus-like particle that lacks genes for its own replication?
A) Conjugation of an F+ plasmid
B) Gene transfer through a gene transfer agent
C) Transduction by a dsDNA phage Mu
D) Transformation of a linear piece of DNA
A plasmid may
A) replicate independently of the chromosome.
B) be transferred cell-to-cell during conjugation.
C) be integrated into the chromosome.
D) replicate independently of the chromosome, integrate into the chromosome, or be transferred cell-to-cell during conjugation.
Chapter 8 Solutions
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
Ch. 8 - Prob. 1SACh. 8 - Why is deleting one nucleotide generally more...Ch. 8 -
3. What type of mutation in an operon is most...Ch. 8 -
4. What is meant by "proofreading" with respect...Ch. 8 - Why would a cell use SOS repair, considering that...Ch. 8 - Why is replica plating used to isolate an...Ch. 8 - What is transduction?Ch. 8 -
8. How is an F+ strain different from an Hfr...Ch. 8 - Name four mobile genetic elements.Ch. 8 - Why are R plasmids important?
Ch. 8 - UV light exposure forms a) covalent bonds between...Ch. 8 -
2. If cells were exposed to UV light, the highest...Ch. 8 -
3. Penicillin enrichment of mutants works on the...Ch. 8 -
4. Mechanisms that repair errors in nucleotide...Ch. 8 -
5. You are trying to isolate a mutant of...Ch. 8 -
6. All plasmids
1) carry genes for antimicrobial...Ch. 8 -
7. Adding DNase to a mixture of donor and...Ch. 8 -
8. An F pilus or its equivalent is essential...Ch. 8 - A plasmid that can replicate in E. coli and...Ch. 8 -
10. The frequency of transfer of an F' molecule...Ch. 8 -
1. Some bacteria may have higher mutation rates...Ch. 8 -
2. A pharmaceutical researcher is disturbed to...Ch. 8 - Prob. 1CTCh. 8 -
2. You have isolated a strain of E. coli that is...
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- In E. coli, the passage of traits from parent to daughter cell occurs via A) transformation B) O integration of a plasmid into the host chromosome C) binary fission D) O horizontal gene transfer E) O Two of the abovearrow_forwardWhich of the following terms describes bacteriophage DNA that has become integrated into the host cell chromosome? A) plasmid B) impolite phage C) transposon D) prophagearrow_forwardWhich of the following enzymes’ combined action leads to the generation of sticky ends in plasmid vector?a) Alkaline phosphatase and terminal transferaseb) Exonuclease III and alkaline phosphatasec) Bacteriophage lambda exonuclease and terminal transferased) Exonuclease III and terminal transferasearrow_forward
- Generally a plasmid vector contains how many elements?a) 1b) 2c) 3d) 4arrow_forward1. What is a plasmid? A) An organelle found in plants that conducts photosynthesis. B) Circular extrachromosomal DNA. C) An essential part of the bacterial chromosome. 2. What is the role of the NaOH in plasmid isolation? A) It increases the pH. B) It denatures the DNA. C) Both of the above. 3. In which direction will the DNA migrate in an agarose gel? A) To the positive pole. B) To the negative pole. C) That depends on the sequence of the DNA. 4. What is the function of SDS? A) It denatures proteins. B) It disintegrates the cell membrane. C) Both of the above. 5. To which species group does E. coli belong to? A) Bacteria, B) Fungi. C) Archaea.arrow_forwardWhat is wrongly matched? A) None of the above B) Transfer of genetic material from 1 cell to --- conjugation. Another involving cell to cell contact C) Transfer of DNA from 1 cell to another by a Bacteriophage---Transduction D) Bacterial cell in which the F factor has become integrated---HFrarrow_forward
- What is the function of a vector?a) Destroys cells that do not contain cloned DNAb) Allows cells to take up foreign DNAc) Carries cloned DNA, allowing it to replicate in cellsd) Encodes herbicide resistancee) Encodes Bt toxinarrow_forwardAll of the following are true statements concerning plasmids EXCEPT: O 1) They can carry genes that confer resistance against antibiotics 2) They can be found in bacteria, fungi, and protozoa 3) They carry genes that are required for growth and repair 4) They are small circular molecules of DNA. O 5) They can replicate autonomouslyarrow_forwardTransfection efficiency refers to a) total number of transfected cells per dish O b) total number of transfected cells per field of view total amount of plasmid DNA added per sample d) number of cells per 100 that have taken up plasmid DNAarrow_forward
- A plasmid that can replicate in E. coli and Pseudomonas is most likely a/ana) broad host range plasmid.b) self-transmissible plasmid.c) high-copy-number plasmid.d) essential plasmid.e) low-copy-number plasmid.arrow_forwardWhich of the following is true about plasmids?a) They are present in all organismsb) They are present in bacteria onlyc) They are present in bacteria and phagesd) They are present in plants and animalsarrow_forwardWhich of the following statements is true about a vector?a) Plasmids, phages can be used as vectorsb) All vectors are plasmidsc) Cyanobacteria can also be used as vectorsd) Fungi can also be used as vectorsarrow_forward
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