NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
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Textbook Question
Chapter 8, Problem 2A
A pharmaceutical researcher is disturbed to discover that the major ingredient of a new drug formulation causes frame-shift mutations in bacteria. What other information would the researcher want before looking for a substitute chemical?
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Chapter 8 Solutions
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
Ch. 8 - Prob. 1SACh. 8 - Why is deleting one nucleotide generally more...Ch. 8 -
3. What type of mutation in an operon is most...Ch. 8 -
4. What is meant by "proofreading" with respect...Ch. 8 - Why would a cell use SOS repair, considering that...Ch. 8 - Why is replica plating used to isolate an...Ch. 8 - What is transduction?Ch. 8 -
8. How is an F+ strain different from an Hfr...Ch. 8 - Name four mobile genetic elements.Ch. 8 - Why are R plasmids important?
Ch. 8 - UV light exposure forms a) covalent bonds between...Ch. 8 -
2. If cells were exposed to UV light, the highest...Ch. 8 -
3. Penicillin enrichment of mutants works on the...Ch. 8 -
4. Mechanisms that repair errors in nucleotide...Ch. 8 -
5. You are trying to isolate a mutant of...Ch. 8 -
6. All plasmids
1) carry genes for antimicrobial...Ch. 8 -
7. Adding DNase to a mixture of donor and...Ch. 8 -
8. An F pilus or its equivalent is essential...Ch. 8 - A plasmid that can replicate in E. coli and...Ch. 8 -
10. The frequency of transfer of an F' molecule...Ch. 8 -
1. Some bacteria may have higher mutation rates...Ch. 8 -
2. A pharmaceutical researcher is disturbed to...Ch. 8 - Prob. 1CTCh. 8 -
2. You have isolated a strain of E. coli that is...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- In the procedure called RNA sequencing (RNA-Seq), what type ofmolecule is actually sequenced?arrow_forwardA mutation is most often cause by which of the following factors? a) Sunlight b) Smoking c) Random Copy error d) Radiationarrow_forwardIdentify which mutation is most likely to impact the function of the protein it encodes a) silent mutation b) mutation at the third site of a codon c) nonsense mutation d) missense mutationarrow_forward
- 35)arrow_forwardIn a sequencing reaction, the dATP was left out of the tube.What would be the result of this error?a) No synthesis would occur.b) Synthesis would never continue past the first A.c) Synthesis would not stop until the end of the template.d) Synthesis would terminate randomly, regardless of the nucleotide incorporated.e) The error would have no effect.arrow_forwardSome bacteria may have higher mutation rates than others following exposure to UV light. Discuss a reason why this might be the case. What experiments could you do to determine whether this is a likely possibility?arrow_forward
- When the Oxford team of Ernst Chain and Nor- man Heatley had laboriously collected their first two grams of penicillin (probably no more than 2% pure!), Chain injected two normal mice with 1 g each of this preparation, and waited to see what would happen. The mice survived with no apparent ill effects. Their boss, Howard Florey, was furious at what he saw as a waste of good antibiotic. Why was this experiment important?arrow_forwardWhy does reversible chain termination sequencing yield short reads?arrow_forwardUsing the question in the image below, identify the locations of each mutation in strains 1, 2, and 3arrow_forward
- If the mutation has a negligible effect on the function of a gene, it is known as aa) Silent mutationb) Frame shift mutationc) Substitution mutationd) Insertion mutationarrow_forwardThe following DNA sequence is from a bacteriophage that infects a pathogenic bacterium and scientists want to know if this bacteriophage could prove to be a potential treatment against it. But first scientists need to discover if different strains of this pathogen have restriction endonucleases that it may use for its own protection. They try 3 different RE’s:a) EcoR1 b) HaeIII c) BamH1 Look up the recognition sequences for the 3 Res. Enzymes above and check whether the phage genome (a snippet of which is shown below) will or will not be ‘cut’. Tell me how their experiment worked out and what their conclusion was.G A A A A G G C C A C A A G G C C G T C G A C T T T T A A A A G G C C A C A T G C G G C T T T T C C G G T G T T C C G G C AG C T GA A A AT T T T C C G G T G T A C G CCarrow_forwardWhy is Sanger sequencing sometimes referred to as "dye-terminator" sequencing?arrow_forward
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