NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
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Textbook Question
Chapter 8, Problem 2MC
If cells were exposed to UV light, the highest frequency of mutations would be obtained if the cells were immediately
a) placed in the dark.
b) exposed to visible light.
c) shaken vigorously.
d) incubated at a temperature below their optimum for growth.
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a) Because only hyperthermophiles have DNA polymerases.
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Chapter 8 Solutions
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
Ch. 8 - Prob. 1SACh. 8 - Why is deleting one nucleotide generally more...Ch. 8 -
3. What type of mutation in an operon is most...Ch. 8 -
4. What is meant by "proofreading" with respect...Ch. 8 - Why would a cell use SOS repair, considering that...Ch. 8 - Why is replica plating used to isolate an...Ch. 8 - What is transduction?Ch. 8 -
8. How is an F+ strain different from an Hfr...Ch. 8 - Name four mobile genetic elements.Ch. 8 - Why are R plasmids important?
Ch. 8 - UV light exposure forms a) covalent bonds between...Ch. 8 -
2. If cells were exposed to UV light, the highest...Ch. 8 -
3. Penicillin enrichment of mutants works on the...Ch. 8 -
4. Mechanisms that repair errors in nucleotide...Ch. 8 -
5. You are trying to isolate a mutant of...Ch. 8 -
6. All plasmids
1) carry genes for antimicrobial...Ch. 8 -
7. Adding DNase to a mixture of donor and...Ch. 8 -
8. An F pilus or its equivalent is essential...Ch. 8 - A plasmid that can replicate in E. coli and...Ch. 8 -
10. The frequency of transfer of an F' molecule...Ch. 8 -
1. Some bacteria may have higher mutation rates...Ch. 8 -
2. A pharmaceutical researcher is disturbed to...Ch. 8 - Prob. 1CTCh. 8 -
2. You have isolated a strain of E. coli that is...
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- A cytosine is deaminated, forming uracil. Which DNA repair systems is best able to correct this error?a) CPD photolyase repairb) mismatch repair c) nucleotide-excision repair d) base-excision repaire) homology-directed repairarrow_forwardSequence of which of the following cannot be determined using the Maxam Gilbert method?a) Bacteriab) Plantsc) Bacteriophage T7d) Plasmidarrow_forward38) The following treatment(s) control bacterial growth by damaging the DNA structure of bacteria: a) Fluroquinolones b) UV light c) Radiation d) Rifamycin e) b & c39) The following treatment(s) stops transcription: a) Fluroquinolones b) UV light c) Radiation d) Rifamycin e) a & b40) Bacteria that grow optimally between the pH 5.5 – 8 are called: a) Acidophiles b) Neutrophiles c) Alkaliphiles d) Hydrophiles e) Nabilophiles41) All the following methods are physical ways for controlling bacterial growth except: a) chemotherapy b) Moist heat c) dry heat d) radiation e) low temperatures.42) Setting the autoclave time at the prescribed temperatures depending of what material you are autoclaving is called: a) D-Value b) Z-Value c) F-Value d) Autoclave Coefficient e) TValue43) A treatment that kills or removes all living cells, including viruses and spores, from a substance or objects is called: a) antiseptic b) sanitization c) disinfection d) chemotherapeutic e) sterilizatioarrow_forward
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- The percentage of the agarose gel is important because a) it influences the rate of migration of the fragments b) it may cause some DNA molecules to replicate c) some DNA nucleotides may degraded due to chemical reactions with the gel d) some DNA molecules may sink to the bottom of the well and not migrate What is the purpose of a buffer used in electrophoresis? a) Increase/decrease the current b) Conduct the current and protect the samples c) Disrupt the bands d) Stop the band migration Which of the following is an additional use of the gel electrophoresis technique? a) To express DNA b) To separate RNA c) To amplify RNA d) To amplify genesarrow_forwardSelect the true statements: A.) It is possible, through genetic engineering, to fuse a fluorescent molecule (example GFP) with a non-fluorescent protein to create a fluorescent fusion protein. B.) Proteins are created during gene transcription. C.) It is possible to observe a fluorescent protein immediately after the plasmid coding for this protein is introduced into a cell. D.) The plasma coding for a fluorescent protein is also fluorescent. E.) It is possible to obtain a different color fluorescent fusion protein by replacing the sequence coding for the fluorescent protein in the plasmid for a sequence coding for a fluorescent protein by a different color sequence.arrow_forwardthe nucleotide excision repair system can undo damaging caused by UV radiation? a) cataracts in lens of the eye b) protein-protein cross-links c) thymine dimers d) alternative splicing errors e) apurinic sitesarrow_forward
- Generally a plasmid vector contains how many elements?a) 1b) 2c) 3d) 4arrow_forwardChemical mutagens often cause oxidation or deamination of DNA bases. This can lead to cancer by causing: A) Activation of replication. B) Activation of transcription. C) Non Watson-Crick base pairing. OD) Changes to cytochrome P450 enzymes. OE) Activation of translation.arrow_forwardWhat are the main components needed for electrophoresis? a) A power source b) A gel matrix c) A medium (or "buffer") d) Casting tray e) All of the above For what purpose is DNA electrophoresis used? a) To amplify a DNA for cloning b) To separate noncharged molecules of DNA based on size c) To separate charged molecules of DNA based on size d) To separate charged molecules of DNA based on amount of positive charge e) To produce static electricity by frictionarrow_forward
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