NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
expand_more
expand_more
format_list_bulleted
Concept explainers
Textbook Question
Chapter 8, Problem 1A
Some bacteria may have higher mutation rates than others following exposure to UV light. Discuss a reason why this might be the case. What experiments could you do to determine whether this is a likely possibility?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
As shown , several medical agents are now commercially produced by genetically engineered microorganisms. Discuss the advantages and disadvantages of making these agents this way.
The following image is of an agarose gel. If DNA samples were loaded to this gel and the electrophoresis experiment was started, explain what would happen and why.
Consider the following experiment. First, large populations of two mutant strains of Escherichia coli are mixed, each requiring a different, single amino acid. After plating them onto a minimal medium, 45 colonies grew. Which of the following may explain this result?
A) The colonies may be due to back mutation (reversion).
B) The colonies may be due to recombination.
C) Either A or B is possible.
D) Neither A nor B is possible.
Chapter 8 Solutions
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
Ch. 8 - Prob. 1SACh. 8 - Why is deleting one nucleotide generally more...Ch. 8 -
3. What type of mutation in an operon is most...Ch. 8 -
4. What is meant by "proofreading" with respect...Ch. 8 - Why would a cell use SOS repair, considering that...Ch. 8 - Why is replica plating used to isolate an...Ch. 8 - What is transduction?Ch. 8 -
8. How is an F+ strain different from an Hfr...Ch. 8 - Name four mobile genetic elements.Ch. 8 - Why are R plasmids important?
Ch. 8 - UV light exposure forms a) covalent bonds between...Ch. 8 -
2. If cells were exposed to UV light, the highest...Ch. 8 -
3. Penicillin enrichment of mutants works on the...Ch. 8 -
4. Mechanisms that repair errors in nucleotide...Ch. 8 -
5. You are trying to isolate a mutant of...Ch. 8 -
6. All plasmids
1) carry genes for antimicrobial...Ch. 8 -
7. Adding DNase to a mixture of donor and...Ch. 8 -
8. An F pilus or its equivalent is essential...Ch. 8 - A plasmid that can replicate in E. coli and...Ch. 8 -
10. The frequency of transfer of an F' molecule...Ch. 8 -
1. Some bacteria may have higher mutation rates...Ch. 8 -
2. A pharmaceutical researcher is disturbed to...Ch. 8 - Prob. 1CTCh. 8 -
2. You have isolated a strain of E. coli that is...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Imagine that you are a student in Alfred Hershey and Martha Chase’s lab in the late 1940s. You are given five test tubes containing E. Coli bacteria infected with T2 bacteriophages that have been labeled with either 32P or 35S. Unfortunately, you forget to mark the tubes and are now uncertain about which tubes is which. You performed their blender experiment and got the following results. Which tube out of these 5 contains E. Coli infected with 32P-labeled phage? Explain your answer.arrow_forwardResearchers are designing several experiments to test the ability of Salmonella bacteria to develop antibiotic resistance. A culture of Salmonella bacteria is exposed to the same concentrations (200 mg/L) of an antibiotic for four days. The table shows the number of isolated resistant bacteria over a four-day period. Which of the following statements best explains these results? A - The bacteria were not affected by the antibiotic. B - After being exposed to the antibiotic, the bacteria altered their DNA. C - A new species of bacteria emerged after the antibiotics were introduced. D - Random mutations led some bacteria to be resistant and, over time, they increased in the population.arrow_forwardSalmonella strains developed for the Ames test contain many special phenotypes that make them more useful for these techniques. Many of these phenotypes, including UvrA and rfa, make these strains mutate more easily, and therefore make the assays more sensitive. In 1-2 sentences each, state how the UvrA and rfa phenotypes make Ames test strains more sensitive to mutations.arrow_forward
- You perform an Ames test to determine which compound X causes mutations or not. You grow an auxotrophic strain of bacteria on three plates; one with no compound X, one with just compound X and one with compound X mixed with liver enzymes. You find many more colonies on the plates with compound X and with compound X plus liver enzymes compared to the control plate. Which of the following describes the best interpretation of the results? Compound X causes mutations on it's own but is safe after exposure to liver enzymes. Compound X is not mutagenic. Compound X is mutagenic regardless of whether it has been processed by liver enzymes. Compound X is mutagenic only after being treated with liver enzymes.arrow_forwardConsidering the proclivity of bacteria to share genetic information, discuss the concerns behind phenomena such as antibiotic resistance. In other words, why might it be a bad idea to not finish your antibiotic therapy as prescribed by your physician? Some people think that "saving some for later" is a good idea. Why might that not be the case?arrow_forwardCystic fibrosis (CF) is a genetic disorder affecting a number of organs, including the lung airways, pancreas, and sweat glands. Mutations in both copies of the CFTR gene causes cystic fibrosis. Imagine that you have sweat gland samples from several Cystic Fibrosis patients (A-C) with unknown mutations in CFTR. You also have normal (+) sweat gland sample to use as a positive control. А В С А В С Choose which mutation would explain the RNA and protein results in A, B, & C: 1. Promoter/Regulatory mutation 2. Silent mutation 3. Missense mutation 4. Deletion mutation 5. Splice site mutation 6. Nonsense mutation RNA gel Protein gelarrow_forward
- A hypothetical gene for cephalosporin resistance is found to be carried by a transposon. Explain what a transposon is. Then explain how the cephalosporin resistance could be horizontally transferred between organisms by transformation, conjugation, and transduction. What steps/events would have to occur to allow the transposon to be transferred by each method. Also, explain how it could be transferred vertically between organisms.arrow_forwardYour TA gives you an Escherichia coli strain (AmreB) that carries a gene deletion in the mreB gene. As a result, the strain is not able to produce the MreB protein. Your task is to compare the morphology of actively growing AmreB cells to the parental wildtype strain (produces MreB). What difference in morphology will you likely observe? Would you expect the same in Staphylococcus aureus?arrow_forwardPlease answer the following questions using numbers and not text. For example, if you think the answer is 24, write 24 and not twenty-four. If you think the answer is 24 ATP, write only the number 24. As a microbiology technician, your job is to characterize a novel bacterial strain isolated from insects. Phenotypically, you have discovered that the new bacterial strain can grow in the presence or absence of oxygen. After genome sequencing and biochemical characterization, you know that your new organism is capable of making all of the enzymes necessary for glycolysis and the TCA cycle, and that it has an electron transport chain. Answer the following questions based on the growth of your organism on minimal media plates containing only acetate as a carbon and energy source. Acetate is transported across the membrane at the cost of 3 ATP per molecule. Each question should be answered based on the metabolism of one molecule of acetate. If you come up with a negative value, indicate an…arrow_forward
- What order should the steps be in for this culture method?arrow_forwardWhen plasmids are isolated from bacterial cells, they may existin a number of forms.a. List the different forms that may be found.b. Which do you think would migrate the fastest and farthest in anelectrophoresis experiment and why?arrow_forwardIn a process of production of a recombinant protein by E. coli cells, it was observed accumulation of acetate in the culture medium. In this situation, it can be said that: (a) certainly the process in question was being conducted in anaerobiosis (B).Acetate accumulation is advantageous for the process as the acetate formation reaction generates 1 molecule of ATP (c)Knowing that decreasing the temperature of the process causes a reduction in the rate of glycolysis, this could be a strategy to reduce the accumulation of acetate (d).the acetate formed can be re-assimilated by the cell if the glyoxylate pathway is activated at some point in the culturearrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license