NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
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Textbook Question
Chapter 8, Problem 9MC
A plasmid that can replicate in E. coli and Pseudomonas is most likely a/an
a) broad host range plasmid.
b) self-transmissible plasmid.
c) high-copy-number plasmid.
d) essential plasmid.
e) low-copy-number plasmid.
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Students have asked these similar questions
A plasmid may
A) replicate independently of the chromosome.
B) be transferred cell-to-cell during conjugation.
C) be integrated into the chromosome.
D) replicate independently of the chromosome, integrate into the chromosome, or be transferred cell-to-cell during conjugation.
1. What is a plasmid?
A) An organelle found in plants that conducts photosynthesis.
B) Circular extrachromosomal DNA.
C) An essential part of the bacterial chromosome.
2. What is the role of the NaOH in plasmid isolation?
A) It increases the pH.
B) It denatures the DNA.
C) Both of the above.
3. In which direction will the DNA migrate in an agarose gel?
A) To the positive pole.
B) To the negative pole.
C) That depends on the sequence of the DNA.
4. What is the function of SDS?
A) It denatures proteins.
B) It disintegrates the cell membrane.
C) Both of the above.
5. To which species group does E. coli belong to?
A) Bacteria,
B) Fungi.
C) Archaea.
Which of the statements is TRUE about conjugation?
a)
Plasmid DNA is transferred from a donor (F+) cell
b)
It may only be performed by competent cells
c)
DNA is always integrated into recipient chromosome
d)
It may be specialized or generalized
Chapter 8 Solutions
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
Ch. 8 - Prob. 1SACh. 8 - Why is deleting one nucleotide generally more...Ch. 8 -
3. What type of mutation in an operon is most...Ch. 8 -
4. What is meant by "proofreading" with respect...Ch. 8 - Why would a cell use SOS repair, considering that...Ch. 8 - Why is replica plating used to isolate an...Ch. 8 - What is transduction?Ch. 8 -
8. How is an F+ strain different from an Hfr...Ch. 8 - Name four mobile genetic elements.Ch. 8 - Why are R plasmids important?
Ch. 8 - UV light exposure forms a) covalent bonds between...Ch. 8 -
2. If cells were exposed to UV light, the highest...Ch. 8 -
3. Penicillin enrichment of mutants works on the...Ch. 8 -
4. Mechanisms that repair errors in nucleotide...Ch. 8 -
5. You are trying to isolate a mutant of...Ch. 8 -
6. All plasmids
1) carry genes for antimicrobial...Ch. 8 -
7. Adding DNase to a mixture of donor and...Ch. 8 -
8. An F pilus or its equivalent is essential...Ch. 8 - A plasmid that can replicate in E. coli and...Ch. 8 -
10. The frequency of transfer of an F' molecule...Ch. 8 -
1. Some bacteria may have higher mutation rates...Ch. 8 -
2. A pharmaceutical researcher is disturbed to...Ch. 8 - Prob. 1CTCh. 8 -
2. You have isolated a strain of E. coli that is...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Which process listed below allows genetic material to be transferred from a virus-like particle that lacks genes for its own replication? A) Conjugation of an F+ plasmid B) Gene transfer through a gene transfer agent C) Transduction by a dsDNA phage Mu D) Transformation of a linear piece of DNAarrow_forwardWhich of the following statements is true about a vector?a) Plasmids, phages can be used as vectorsb) All vectors are plasmidsc) Cyanobacteria can also be used as vectorsd) Fungi can also be used as vectorsarrow_forwardWhich of the following statements is not applicable to conjugation? A) OF factors are transferrable plasmids passed from one cell to another during conjugation. B) O During conjugation, rolling circle replication transfers an F factor from an F+ cell to an F- cell. ) O An F factor acquired during conjugation will automatically be integrated into the recipient's chromosome. D) O Conjugation requires physical contact between cells via the formation of a sex pilus. E) O An Hfr strain is capable of transferring a portion of the chromosome during conjugation.arrow_forward
- Which of the following statements is false?a) The cells which take up recombinant plasmids are drug resistantb) The cells with no recombinant plasmids are drug resistantc) Plasmid replication is independent of cell’s divisiond) The cells containing recombinant plasmid are called transformed cellsarrow_forwardWhich of the following terms describes bacteriophage DNA that has become integrated into the host cell chromosome? A) plasmid B) impolite phage C) transposon D) prophagearrow_forwardWhich of the following enzymes’ combined action leads to the generation of sticky ends in plasmid vector?a) Alkaline phosphatase and terminal transferaseb) Exonuclease III and alkaline phosphatasec) Bacteriophage lambda exonuclease and terminal transferased) Exonuclease III and terminal transferasearrow_forward
- Generally a plasmid vector contains how many elements?a) 1b) 2c) 3d) 4arrow_forwardTransfection efficiency refers to a) total number of transfected cells per dish O b) total number of transfected cells per field of view total amount of plasmid DNA added per sample d) number of cells per 100 that have taken up plasmid DNAarrow_forwarda) Where is the F plasmid located in an Hfr cell? Where is the F plasmid located in an F+ cell? b)arrow_forward
- Which of the following is NOT a mechanism by which plasmids inerease the possibility of being present in the new generation of cells? A) O They can be present at a high number so that some copies will end up in each new cell after cell division. B) O They encode traits such as antibiotic resistance that are required for growth in some environments. C) O They cause the cell to replicate much more rapidly than cells that do not have plasmids. D) O They may carry host survival genes and self-preservation genes.arrow_forwardYou want to propagate large amounts of a DNA fragment. To do this, use a plasmid vector. a) How should this plasmid vector be constructed? b) Also describe the function of the components.arrow_forwardThe addition of DNase to a mixture of donor and recipientcells will prevent gene transfer viaa) DNA transformation.b) chromosome transfer by conjugation.c) plasmid transfer by conjugation.d) generalized transduction.arrow_forward
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