NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
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Chapter 8, Problem 1CT
Summary Introduction
To analyze:
The type of mutation preferable for the reversion in Ames test: deletion, point mutation, or a frameshift mutation.
Introduction:
Mutations are the permanent alteration in the
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You have a patient in your clinic presenting symptoms of cystic fibrosis. You screen their CFTR gene for mutations, and find the following list:
CFTR 320 L V
CFTR 341 S W
CFTR 528 E D
CFTR 976 F Q
CFTR 1235 S R
Which mutation(s) are likely causing cystic fibrosis in this patient?
You also sequence a newborn family member of this patient. They have all of these same mutations, other than the one at position 976, and no other mutations in CFTR. Do you predict this person will develop cystic fibrosis? Explain why.
Cystic fibrosis (CF) is a genetic disorder affecting a number of organs, including
the lung airways, pancreas, and sweat glands.
Mutations in both copies of the CFTR gene causes cystic fibrosis.
Imagine that you have sweat gland samples from several Cystic Fibrosis patients
(A-C) with unknown mutations in CFTR.
You also have normal (+) sweat gland sample to use as a positive control.
А В С
А В С
Choose which mutation would explain the
RNA and protein results in A, B, & C:
1. Promoter/Regulatory mutation
2. Silent mutation
3. Missense mutation
4. Deletion mutation
5. Splice site mutation
6. Nonsense mutation
RNA gel
Protein gel
Suppose that you could undergo genetic testing at age 18 for susceptibility to a genetic disease that would not appear until middle age and has no available treatment. a. What would be some of the possible reasons for having such a genetic test and some of the possible reasons for not having the test? b. Would you personally want to be tested? Explain your reasoning.
Chapter 8 Solutions
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
Ch. 8 - Prob. 1SACh. 8 - Why is deleting one nucleotide generally more...Ch. 8 -
3. What type of mutation in an operon is most...Ch. 8 -
4. What is meant by "proofreading" with respect...Ch. 8 - Why would a cell use SOS repair, considering that...Ch. 8 - Why is replica plating used to isolate an...Ch. 8 - What is transduction?Ch. 8 -
8. How is an F+ strain different from an Hfr...Ch. 8 - Name four mobile genetic elements.Ch. 8 - Why are R plasmids important?
Ch. 8 - UV light exposure forms a) covalent bonds between...Ch. 8 -
2. If cells were exposed to UV light, the highest...Ch. 8 -
3. Penicillin enrichment of mutants works on the...Ch. 8 -
4. Mechanisms that repair errors in nucleotide...Ch. 8 -
5. You are trying to isolate a mutant of...Ch. 8 -
6. All plasmids
1) carry genes for antimicrobial...Ch. 8 -
7. Adding DNase to a mixture of donor and...Ch. 8 -
8. An F pilus or its equivalent is essential...Ch. 8 - A plasmid that can replicate in E. coli and...Ch. 8 -
10. The frequency of transfer of an F' molecule...Ch. 8 -
1. Some bacteria may have higher mutation rates...Ch. 8 -
2. A pharmaceutical researcher is disturbed to...Ch. 8 - Prob. 1CTCh. 8 -
2. You have isolated a strain of E. coli that is...
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- Your research lab is interested in determining the phenotype of three of your patients (DNA 1, DNA 2, and DNA 3) for the gene TNF alpha, known to exist in two isoforms (wild-type TNF1 and mutant TNF2). Using gel electrophoresis to isolate the patients DNA, you obtain the results of the experiment as depicted in the left image below. Interested in your results, your boss asks you to perform the experiment again, to ensure your results are consistent. Upon performing the experiment again, you obtain the results as depicted in the right image below.. Assuming the left image is what you should have obtained, which of the following conclusions is correct and indicates potential errors that could have occurred in the right image. You accidentally added the TNF1 and TNF2 control DNA samples to the TNF1 lane, the TNF2 lane incorrectly added TNF2 control only, you forgot to add DNA 2 sample to the DNA 1 lane, you accidentally added DNA 1 sample to the DNA 2 lane, and you accidentally added DNA…arrow_forwardExplain the rationale behind the use of the “bag of worms” phenotype as a way to identify heterochronic mutations.arrow_forwardA genetic variant is associated with high penetrance and low expressivity. This means the variant is associated with a: Question 15 options: High chance of showing disease symptoms and the disease is caused by a rare mutation. High chance of showing disease symptoms but the symptoms will be mild. Low chance of showing disease symptoms and the symptoms will be mild. High chance of showing disease symptoms and the symptoms will be severe. Low chance of showing disease symptoms but the symptoms will be severe.arrow_forward
- Explain the Detection of the sickle-cell mutation by sequencing of PCR products?arrow_forwardThe table below shows different types of mutations in different positions in four genes. Choose the letter (A to E), from the drop-down menu, that represents the most likely type of protein that will be produced from each of these mutated genes. A: completely normal protein B: functional protein with ONE amino acid different from normal C: non-functional protein with ONE amino acid different from normal D: non-functional protein with MANY amino acids different from normal E: no protein at all Answer Type of mutation Position of mutation in gene (A, B, C, D, or E) before the part of the gene that specifies the active site of the enzyme 2 base pair insertion Inonsense immediately before the stop codon in the part of the gene that specifies the active site of the enzyme silent 1 base pair insertion in an intronarrow_forwardI have complete the table that required. Indicate which mutations were success why some were not from the size about. indicate at what stage any of the mutagenesis failed.arrow_forward
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