NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
9th Edition
ISBN: 9781265432287
Author: Anderson
Publisher: MCG CUSTOM
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Chapter 8, Problem 3MC
Penicillin enrichment of mutants works on the principle that the antibiotic
a) kills only Gram-positive cells.
b) primarily kills cells during the
c) kills most Gram-negative cells.
d) kills only growing cells.
e) inhibits formation of the lipopolysaccharide layer.
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Chapter 8 Solutions
NESTER'S MICROBIOLOGY-CONNECT >CUSTOM<
Ch. 8 - Prob. 1SACh. 8 - Why is deleting one nucleotide generally more...Ch. 8 -
3. What type of mutation in an operon is most...Ch. 8 -
4. What is meant by "proofreading" with respect...Ch. 8 - Why would a cell use SOS repair, considering that...Ch. 8 - Why is replica plating used to isolate an...Ch. 8 - What is transduction?Ch. 8 -
8. How is an F+ strain different from an Hfr...Ch. 8 - Name four mobile genetic elements.Ch. 8 - Why are R plasmids important?
Ch. 8 - UV light exposure forms a) covalent bonds between...Ch. 8 -
2. If cells were exposed to UV light, the highest...Ch. 8 -
3. Penicillin enrichment of mutants works on the...Ch. 8 -
4. Mechanisms that repair errors in nucleotide...Ch. 8 -
5. You are trying to isolate a mutant of...Ch. 8 -
6. All plasmids
1) carry genes for antimicrobial...Ch. 8 -
7. Adding DNase to a mixture of donor and...Ch. 8 -
8. An F pilus or its equivalent is essential...Ch. 8 - A plasmid that can replicate in E. coli and...Ch. 8 -
10. The frequency of transfer of an F' molecule...Ch. 8 -
1. Some bacteria may have higher mutation rates...Ch. 8 -
2. A pharmaceutical researcher is disturbed to...Ch. 8 - Prob. 1CTCh. 8 -
2. You have isolated a strain of E. coli that is...
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- Identify the most mistaken (wrong) choice: a) Transcription machinery and an enhancer can bind to the chromosome at the same time. b) Organic matters may interfere with heat treatment of bacterial growth control. ( c) Nitrocellulose can be used to filter out microorganisms from a liquid solution. d) Time to kill a bacterial culture is not proportional to the number of microbes in the culturearrow_forwardWhich of the following best explains why there is no growth on plate II? (a) The initial bacterial culture was not ampicillin-resistant. (b) The transformation procedure killed the bacteria. (c) Nutrient agar inhibits bacterial growth. (d) The bacteria on the plate were transformed. (e) The initial bacterial culture is ampicillin-resistant.arrow_forwardYou genetically engineer nonadhesive cells to express one variety of cadherins and then mix the cells in various combinations. You then monitor their interactions. What do you observe?a) The cells adhered preferentially to cells expressing one of the other cadherins.b) The engineered cells adhered preferentially to cells expressing the same cadherins.c) The genetically engineered cells did not stick to any other cells.d) The genetically engineered cells died.e) The genetically engineered cells migrated to a different location.arrow_forward
- Why must the top agar be supplemented with MgSo4 A) it is required to induce the production of cro repressors B) it is required to induce the production of cI repressors C) it helps stabilize the phage for infection D) it induces the expression of the lamB gene in E. Coliarrow_forwardWe have two specific strains of E. coli that have shown horizontal gene transfer (HGT) when mixed. To experimentally determine the method of HGT that is happening, the following conditions are set up in different tubes of culture media: A) Donor and recipient strain mixed together (control - no treatment). B) Donor and recipient strains mixed together, DNase added (can digest DNA in solution, not within cells).C) Special tube containing a membrane filter (with pores that allow DNA and viruses to pass through, but not bacterial cells) that separates two compartments. Donor strain is added on one side, the recipient strain on the other (they are separated by the filter).D) Donor and recipient strains mixed together, with chemical that inactivates viruses (chemical affects bacteriophages in solution so they are unable to attach to cells). The results: Tubes A, B, and D: HGT was observed. Tube C: HGT was NOT observed. Based on this, which type of HGT was occurring? Conjugation,…arrow_forwardWhich of the following does not act as a restriction enzyme?a) EcorIb) BamHIc) HindIIId) polydeoxyribonucleotide synthasearrow_forward
- Which of the following mechanisms will remove uracil and incorporate the correct base?a) Direct repairb) Base excision repairc) Mismatch repaird) Nucleotide excision repairarrow_forwardIn conjugation of a Hfr cell with an F- cell: a) the container cell becomes F + b) The container cell remains F- c) The donor cell becomes F- d) The recipient cell becomes Hfrarrow_forwardVarious antimicrobial drugs to treat microbial infection have diverse mechanism of action. Consider the following antimicrobial drugs: A. Seconeolitsine, known as DNA topoisomerase I inhibitor in bacteria. (i) Explain briefly how inhibiting DNA topoisomerase I is a good mechanism of action for an antibiotic, include possible molecular machineries being targeted. (ii) What would be an appropriate response if seconeolitsine works well by stating the state of supercoiling in bacteria. (iii) To prove your answer (ii), you test the condition of bacterial DNA by running gel electrophoresis, one has been treated with seconeolitsine (+ sample) and the other one is not (- sample). Explain the position of each + sample and – sample band on the gel in reference to the point of origin (where you load your samples) or how far each DNA sample travel across agarose gel. (iv) Explain why you would expect answer (iii) for each + sample and – sample. B.…arrow_forward
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