Biochemistry
9th Edition
ISBN: 9781305961135
Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher: Cengage Learning
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Chapter 8, Problem 48RE
Interpretation Introduction
Interpretation:
The reason for the fact that overdoses of lipid soluble vitamins can cause toxicity in the body, is to be provided.
Concept introduction:
Vitamins are small organic compounds that are required in very small quantity and are not synthesized by the body. They have to be obtained through diet.
Vitamins are majorly classified into two groups: fat-soluble and water-soluble vitamins. Fat-soluble vitamins include vitamin A, vitamin D, vitamin E, and vitamin K.
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 8 Solutions
Biochemistry
Ch. 8 - RECALL Proteins, nucleic acids, and carbohydrates...Ch. 8 - RECALL What structural features do a...Ch. 8 - RECALL Draw the structure of a phosphoacylglycerol...Ch. 8 - RECALL What structural features do a sphingomyelin...Ch. 8 - RECALL You have just isolated a pure lipid that...Ch. 8 - RECALL What structural features does a...Ch. 8 - RECALL Write the structural formula for a...Ch. 8 - RECALL How does the structure of steroids differ...Ch. 8 - Prob. 9RECh. 8 - REFLECT AND APPLY Which is more hydrophilic,...
Ch. 8 - Prob. 11RECh. 8 - REFLECT AND APPLY Succulent plants from arid...Ch. 8 - REFLECT AND APPLY In the produce department of...Ch. 8 - REFLECT AND APPLY Egg yolks contain a high amount...Ch. 8 - REFLECT AND APPLY In the preparation of sauces...Ch. 8 - REFLECT AND APPLY When water birds have had their...Ch. 8 - RECALL Which of the following lipids are not found...Ch. 8 - RECALL Which of the following statements is (are)...Ch. 8 - REFLECT AND APPLY Why might some food companies...Ch. 8 - Prob. 20RECh. 8 - BIOCHEMICAL CONNECTIONS Crisco is made from...Ch. 8 - BIOCHEMICAL CONNECTIONS Why does the American...Ch. 8 - REFLECT AND APPLY In lipid bilayers, there is an...Ch. 8 - Prob. 24RECh. 8 - REFLECT AND APPLY Suggest a reason why the cell...Ch. 8 - REFLECT AND APPLY Suggest a reason why animals...Ch. 8 - REFLECT AND APPLY What is the energetic driving...Ch. 8 - Prob. 28RECh. 8 - RECALL How can fluorescence techniques be used to...Ch. 8 - Prob. 30RECh. 8 - Prob. 31RECh. 8 - REFLECT AND APPLY A membrane consists of 50%...Ch. 8 - REFLECT AND APPLY Suggest a reason why the same...Ch. 8 - REFLECTANDAPPLY Suppose that you are studying a...Ch. 8 - REFLECT AND APPLY Which statements are consistent...Ch. 8 - RECALL What role does phosphorylation of tyrosine...Ch. 8 - Prob. 37RECh. 8 - REFLECT AND APPLY Suggest a reason why inorganic...Ch. 8 - REFLECT AND APPLY Which statements are consistent...Ch. 8 - RECALL What happens to the human growth hormone...Ch. 8 - Prob. 41RECh. 8 - RECALL What is the structural relationship between...Ch. 8 - Prob. 43RECh. 8 - RECALL What are isoprene units? What do they have...Ch. 8 - Prob. 45RECh. 8 - Prob. 46RECh. 8 - Prob. 47RECh. 8 - Prob. 48RECh. 8 - Prob. 49RECh. 8 - Prob. 50RECh. 8 - REFLECT AND APPLY A health-conscious friend asks...Ch. 8 - Prob. 52RECh. 8 - Prob. 53RECh. 8 - Prob. 54RECh. 8 - REFLECT AND APPLY List two classes of compounds...Ch. 8 - BIOCHEMICAL CONNECTIONS Outline a possible...
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