Biochemistry
9th Edition
ISBN: 9781305961135
Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher: Cengage Learning
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 15, Problem 10RE
MATHEMATICAL Calculate
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 15 Solutions
Biochemistry
Ch. 15 - RECALL Is there a connection between the...Ch. 15 - REFLECT AND APPLY What do the following indicators...Ch. 15 - REFLECT AND APPLY Consider the reaction...Ch. 15 - RECALL What conditions are necessary for the...Ch. 15 - REFLECT AND APPLY Why is it important that energy...Ch. 15 - RECALL Why is it necessary to define a modified...Ch. 15 - RECALL Which of the following statements is (are)...Ch. 15 - RECALL How can you tell if the standard Gibbs free...Ch. 15 - RECALL Can the thermodynamic property G be used to...Ch. 15 - MATHEMATICAL Calculate G for the following values...
Ch. 15 - Prob. 11RECh. 15 - MATHEMATICAL Consider the reaction AB+C, where...Ch. 15 - Prob. 13RECh. 15 - MATHEMATICAL The G for the reaction Citrate ...Ch. 15 - MATHEMATICAL If a reaction can be written AB, and...Ch. 15 - Prob. 16RECh. 15 - Prob. 17RECh. 15 - Prob. 18RECh. 15 - RECALL Organize the following words into two...Ch. 15 - Prob. 20RECh. 15 - REFLECT AND APPLY Would you expect the production...Ch. 15 - Prob. 22RECh. 15 - REFLECT AND APPLY Adult humans synthesize large...Ch. 15 - RECALL Identify the molecules oxidized and reduced...Ch. 15 - RECALL For each of the reactions in Question 24,...Ch. 15 - Prob. 26RECh. 15 - RECALL What is the structural difference between...Ch. 15 - RECALL How does the difference between NADH and...Ch. 15 - RECALL Which coenzyme is a reactant in the...Ch. 15 - Prob. 30RECh. 15 - Prob. 31RECh. 15 - Prob. 32RECh. 15 - REFLECT AND APPLY The following half reactions...Ch. 15 - Prob. 34RECh. 15 - REFLECT AND APPLY There is a reaction in...Ch. 15 - REFLECT AND APPLY There is a reaction in which...Ch. 15 - Prob. 37RECh. 15 - Prob. 38RECh. 15 - Prob. 39RECh. 15 - Prob. 40RECh. 15 - MATHEMATICAL Using the data in Table 15.1,...Ch. 15 - Prob. 42RECh. 15 - Prob. 43RECh. 15 - MATHEMATICAL The standard free-energy change for...Ch. 15 - Prob. 45RECh. 15 - Prob. 46RECh. 15 - Prob. 47RECh. 15 - REFLECT AND APPLY Would you expect an increase or...Ch. 15 - REFLECT AND APPLY Explain and show why...Ch. 15 - Prob. 50RECh. 15 - Prob. 51RECh. 15 - Prob. 52RECh. 15 - Prob. 53RECh. 15 - REFLECT AND APPLY Why are thioesters considered...Ch. 15 - Prob. 55RECh. 15 - REFLECT AND APPLY This is a conjectural question:...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.Similar questions
- Which type of enzyme catalyses the following reaction? oxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase.arrow_forward+NH+ CO₂ +P H₂N + ATP H₂N NH₂ +ADParrow_forwardWhich type of enzyme catalyses the following reaction? oxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase.arrow_forward
- Which features of the curves in Figure 30-2 indicates that the enzyme is not consumed in the overall reaction? ES is lower in energy that E + S and EP is lower in energy than E + P. What does this tell you about the stability of ES versus E + S and EP versus E + P.arrow_forwardLooking at the figure 30-5 what intermolecular forces are present between the substrate and the enzyme and the substrate and cofactors.arrow_forwardprovide short answers to the followings Urgent!arrow_forward
- Pyruvate is accepted into the TCA cycle by a “feeder” reaction using the pyruvatedehydrogenase complex, resulting in acetyl-CoA and CO2. Provide a full mechanismfor this reaction utilizing the TPP cofactor. Include the roles of all cofactors.arrow_forwardB- Vitamins are converted readily into important metabolic cofactors. Deficiency inany one of them has serious side effects. a. The disease beriberi results from a vitamin B 1 (Thiamine) deficiency and ischaracterized by cardiac and neurological symptoms. One key diagnostic forthis disease is an increased level of pyruvate and α-ketoglutarate in thebloodstream. How does this vitamin deficiency lead to increased serumlevels of these factors? b. What would you expect the effect on the TCA intermediates for a patientsuffering from vitamin B 5 deficiency? c. What would you expect the effect on the TCA intermediates for a patientsuffering from vitamin B 2 /B 3 deficiency?arrow_forwardDraw the Krebs Cycle and show the entry points for the amino acids Alanine,Glutamic Acid, Asparagine, and Valine into the Krebs Cycle - (Draw the Mechanism). How many rounds of Krebs will be required to waste all Carbons of Glutamic Acidas CO2?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Cengage Learning
Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY