Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 14, Problem 4P
Identify similarities and differences between an inducible operon and a repressible operon in terms of
a. the transcription
b. the presence and action of allosteric regulatory molecules.
c. the organization of structural genes of the operon.
Expert Solution & Answer
Trending nowThis is a popular solution!
Students have asked these similar questions
a. What is the function of operons in bacterial gene regulation?
b. Describe how a bacterial operon can be regulated by repressible proteins (such as the tryptophan operon). Include in your description both the “on” and “off” states of the operon. Key elements of your diagram should include: Co-repressor, Genes, mRNA, Operator, Operon, Promoter, Repressor, RNA polymerase.
B. A researcher was trying to determine whether two molecules (A and B) were corepressors or inducers in their respective
operon systems. Data was collected regarding the levels of protein and the amount of gene transcription for the genes in
their respective operons. The data is shown below:
Level of protein
High
Transcription of gene 1
Transcription of gene 2
Molecule A
Low
Low
High
High
low
High
High
Low
Molecule B
High
low
Low
a) Dete
b) Identify which system resembles the lac operon system and which resembles the trp operons system
nine whether molecule A and molecule B are
ducers or corepressors. Justify your answers.
Tryptophan (Trp) attenuation is an extra mechanism of regulation of trp operon when trp level is too high. Attenuation causes premature transcription termination.
c) Review the region that is responsible for trp attenuation during low trp level.
Chapter 14 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 14 - 12.1 Bacterial genomes frequently contain groups...Ch. 14 - Transcriptional regulation of operon gene...Ch. 14 - Why is it essential that bacterial cells be able...Ch. 14 - Identify similarities and differences between an...Ch. 14 - The transcription of -galactosidase and permease...Ch. 14 - 12.6 Is attenuation the product of an allosteric...Ch. 14 - The trpL region contains four repeated DNA...Ch. 14 - The CAP binding site in the lac promoter is the...Ch. 14 - What role does cAMP play in transcription of lac...Ch. 14 - How would a cap- mutation that produces an...
Ch. 14 - Explain the circumstances under which attenuation...Ch. 14 - Consider the transcription of genes of the...Ch. 14 - Describe the lytic and lysogenic life cycles of ...Ch. 14 - 12.14 Define antisense RNA, and describe how it...Ch. 14 - 12.15 Attenuation of trp operon transcription is...Ch. 14 - 12.16 In the lac operon, what are the likely...Ch. 14 - Identify which of the following lac operon haploid...Ch. 14 - Prob. 18PCh. 14 - 12.19 List possible genotypes for lac operon...Ch. 14 - Suppose each of the genotypes you listed in parts...Ch. 14 - 12.21 Four independent mutants (mutants A to D)...Ch. 14 - Suppose the lac operon partial diploid...Ch. 14 - Prob. 23PCh. 14 - 12.24 A repressible operon system, like the trp...Ch. 14 - 12.25 What is the likely effect of each of the...Ch. 14 - 12.26 Suppose that base substitution mutations...Ch. 14 - 12.27 Two different mutations affect. Mutant...Ch. 14 - How would mutations that inactivate each of the...Ch. 14 - The bacterial insertion sequence IS 10 uses...Ch. 14 - 12.34 Northern blot analysis is performed on...Ch. 14 - 12.37 The electrophoresis gel shown in part (a) is...Ch. 14 - Prob. 32PCh. 14 - The following hypothetical genotypes have genes A,...Ch. 14 - For an E. coli strain with the lac operongenotype...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Tryptophan (Trp) attenuation is an extra mechanism of regulation of trp operon when trp level is too high. Attenuation causes premature transcription termination. b) Predict what will happen if trp repressor lose its allosteric effect.arrow_forward1) A. What is the difference between a repressible vs, an inducible operon? B. Using diagrams and words, describe how the Lac Operon is regulated by different levels of lactose and glucose. Be sure to show what’s happening at the molecular level, including the roles of lactose, glucose the repressor protein, cAMP and CAP.arrow_forward. If a particular operon encodes enzymes for makingan essential amino acid and is regulated like thetrp operon, then(A) the amino acid inactivates the repressor.(B) the repressor is active in the absence of theamino acid.(C) the amino acid acts as a corepressor.(D) the amino acid turns on transcription of the operonarrow_forward
- The lactose operon in E. coli comprises two structural genes and three regulatory components.a.State the name of each structural gene and describe its function: b.) State the name of each regulatory component and describe its functionarrow_forwardControl of the trp operon relies on which of the following: a. High levels of tryptophan causing a pause (but not termination) in transcription b. Low levels of tryptophan causing a pause (but not termination) in transcription c. Allolactose binding to the inhibitor to prevent it from binding to the promoter d. Tryptophan binding to the inhibitor to prevent it from binding to the promoterarrow_forwardIn the regulation of the tryophan operon in bacteria, the co-occurence of transcription and translation plays a key role regulating full activation of operon expression. Diagram and explain how during the conditions of high tryptophan concentration this system regulates the expression of genes in the operon.arrow_forward
- A mutation that inactivates transcription and translation from the regulatory gene of an inducible operon could result in: Group of answer choices A inactivation of RNA polymerase. B irreversible binding of the repressor to the operator. C three of these choices are correct D inhibition of transcription of the structural genes within the operon. E two of these choices are correct F transcription of the structural genes within that operon.arrow_forwardPredict the effect on the activity of the lac operon of a mutation that disrupts the function of: a) the cap gene, which encodes the CAP protein. b) the CAP-binding site.arrow_forwardThe trp operon, found in E. coli, is a group of genes that encode biosynthetic enzymes for the amino acid tryptophan. (i) Draw a schematic diagram showing the components involved in regulation of the tryptophan operon. (ii) How does the regulation of the trp operon differ from the regulation of the lac operon? Explain.arrow_forward
- Examine the following theoretical operon (gen) in E. coli which contains structural genes (genC, genB, genA) that are involved in amino acid biosynthesis. The gen operon also includes the gene encoding for the regulator molecule within it. When the amino acid that this operon helps synthesize is present, it binds to the regulator forming a complex which can bind to the operator region. Assume that the regulator molecule only binds to the operator in the presence of the amino acid. Question 1 Promoter Operator genC genB gen operon Regulator genA Transcription occurs when: A. The amino acid is not bound to the regulator, causing the regulator to bind to the operator. B. The amino acid is bound to the regulator, causing the regulator to bind to the operator. C. The amino acid is not bound to the regulator, causing the regulator to fail to bind to the operator. D. The amino acid is bound to the regulator, causing the regulator to fail to bind to the operator. E. The operon is…arrow_forwardExamine the following theoretical operon (gen) in E. coli which contains structural genes (genC, genB, genA) that are involved in amino acid biosynthesis. The gen operon also includes the gene encoding for the regulator molecule within it. When the amino acid that this operon helps synthesize is present, it binds to the regulator forming a complex which can bind to the operator region. Assume that the regulator molecule only binds to the operator in the presence of the amino acid. Promoter || Operator genC genB gen operon Regulator genAarrow_forwardThe map of the lac operon is: POZY The promoter (P) region is the start site of transcription through the binding of the RNA polymerase molecule before actual MRNA production. Mutationally altered promoters (P') cannot bìnd the RNA polymerase molecule. Certain predictions can be made about the effect of P mutations. Use your knowledge of the lactose system to complete the table below. Insert a "+" where enzyme is produced and a "-" where enzyme is not produced. Indicate whether the partial diploid strain is lac* (able to grow on lactose-only medium) or lac( cannot grow in lactose medium). B-Galactosidase Permease Phenotype (Lac or Number Genotype No Lactose Lactose No Lactose Lactose Lac) P*O*Z*Y# Example lac+ I*p*O*z*Y* a Ip*o+z*Y* I*p*oCz*Y C I*p*o+z*Yarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Biology (MindTap Course List)BiologyISBN:9781337392938Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. BergPublisher:Cengage Learning
Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning
Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license