Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 14, Problem 13P
Describe the lytic and lysogenic life cycles of
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Suppose you had a bacteriophage λ in which the gene encoding the cro protein had been mutated, so that it had an unusually high affinity for pL - in this phage the first promoter repressed by cro would be pL, not pRM. What effect, if any, would this mutation, and the consequent rapid repression of transcription from pL, have on the outcome of a cell being infected by this mutant bacteriophage? Explain why you would predict that outcome of aninfection by this mutant phage.
Describe the important events that occur during gene transcription in bacteria. What proteins play critical roles in the three stages?
The figure above shows a schematic of genes and transcription control elements from phage λ. Use this figure as an aid to help you describe the molecular events involved in:
a) The establishment of lysogeny
b) The establishment of a lytic life cycle
Chapter 14 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 14 - 12.1 Bacterial genomes frequently contain groups...Ch. 14 - Transcriptional regulation of operon gene...Ch. 14 - Why is it essential that bacterial cells be able...Ch. 14 - Identify similarities and differences between an...Ch. 14 - The transcription of -galactosidase and permease...Ch. 14 - 12.6 Is attenuation the product of an allosteric...Ch. 14 - The trpL region contains four repeated DNA...Ch. 14 - The CAP binding site in the lac promoter is the...Ch. 14 - What role does cAMP play in transcription of lac...Ch. 14 - How would a cap- mutation that produces an...
Ch. 14 - Explain the circumstances under which attenuation...Ch. 14 - Consider the transcription of genes of the...Ch. 14 - Describe the lytic and lysogenic life cycles of ...Ch. 14 - 12.14 Define antisense RNA, and describe how it...Ch. 14 - 12.15 Attenuation of trp operon transcription is...Ch. 14 - 12.16 In the lac operon, what are the likely...Ch. 14 - Identify which of the following lac operon haploid...Ch. 14 - Prob. 18PCh. 14 - 12.19 List possible genotypes for lac operon...Ch. 14 - Suppose each of the genotypes you listed in parts...Ch. 14 - 12.21 Four independent mutants (mutants A to D)...Ch. 14 - Suppose the lac operon partial diploid...Ch. 14 - Prob. 23PCh. 14 - 12.24 A repressible operon system, like the trp...Ch. 14 - 12.25 What is the likely effect of each of the...Ch. 14 - 12.26 Suppose that base substitution mutations...Ch. 14 - 12.27 Two different mutations affect. Mutant...Ch. 14 - How would mutations that inactivate each of the...Ch. 14 - The bacterial insertion sequence IS 10 uses...Ch. 14 - 12.34 Northern blot analysis is performed on...Ch. 14 - 12.37 The electrophoresis gel shown in part (a) is...Ch. 14 - Prob. 32PCh. 14 - The following hypothetical genotypes have genes A,...Ch. 14 - For an E. coli strain with the lac operongenotype...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- As shown a genetic switch that controls the choicebetween the lytic and lysogenic cycles of phage λ. What is agenetic switch? Compare the roles of a genetic switch and a simpleoperator site (like the one found in the lac operon) in generegulation.arrow_forwardDiscuss the genetic switch that controls the lytic versus lysogenic path in bacteriophage lambda.arrow_forwardThe cI protein in bacteriophage l (lambda) is a transcriptional regulator - name the promoters in the phage genome that are regulated by this protein, and give the effect of the cI protein on each promoter you name (i.e. is the cI protein an activator or a repressor for that promoter).arrow_forward
- Illustrate how RAD51 promotes single strand invasion after releasing SPO11-linked oligonucleotides.arrow_forwardThe bacteriophage genome consists primarily of genes encodingproteins that make up the head, collar and tail, and tail fibers When these genes are transcribed following phage infection, howare these proteins synthesized, since the phage genome lacksgenes essential to ribosome structure?arrow_forwardEukaryotes modify mRNA with an m7G cap: an N7-methylated guanosine linked via a 5′ to 5′ triphosphate linkage, and by methylation at the 2'-hydroxyl of the penultimate nucleoside. What cofactors involved in the capping reactionarrow_forward
- Create a concept map that illustrates transcription in eukaryotes by including the following terms: eukaryotic transcription, RNA polymerase I, RNA polymerase II, RNA polymerase III, transcription factors, basal transcription apparatus, regulatory promoter, core promoter, enhancer, TATA box.arrow_forwardThe bacteriophage genome consists of many genes encoding proteins that make up the head, collar, tail, and tail fibers. When these genes are transcribed following phage infection, how are these proteins synthesized, since the phage genome lacks genes essential to ribosome structure?arrow_forwardGive typing answer with explanation and conclusion a) List three eukaryotic gene expression mechanisms that do not occur in prokaryotes. For two of these, give specific examples and the functional outcomes. b) Describe what is meant by the term “RNA silencing”. c) Using diagrams, give two examples of RNA silencing mechanisms and indicate one difference.arrow_forward
- In immediate-early transcription of the phage genome, the N protein works as an antiterminator to allow transcription of the delayed early genes. After this, if the bacterial cell that this lambda phage infects is robustly healthy, it will make many proteases that overwhelm the efforts of the clll protein in protecting the cll protein from degradation. If the cll protein is degraded, protein will block transcription from the PRM promoter and promote the lysis the cro pathway. If the lysogenic pathway prevails, the cll protein is protected P-RM promoter. This results in the which blocks transcription from P-RM cro . Upon integration, the prop until environmental conditions induce the lytic pathway. [Select] cl cill cro N P-L P-RM P-RE transcription from the protein being made, nscription from s host chromosomearrow_forwardWhich of the following is the correct order of Rho-independent termination in prokaryotes? O Transcription of six adenines and rapid folding of RNA into hairpin, transcription of inverted sequences, release of transcript O Transcription of inverted sequences, transcription of six adenines and rapid folding of RNA into hairpin, release of transcript O Transcription of inverted sequences, release of transcript, transcription of six adenines and rapid folding of RNA into hairpin O Transcription of six adenines and rapid folding of RNA into hairpin, release of transcript, transcription of inverted sequencesarrow_forwardThe map of the lac operon is shown below. Consider the following examples that include both haploids and partial diploids and explain in each scenario whether the repressor can bind and regulate expression and whether or not the lac operon is expressed. For partial diploids the plasmid is indicated by the F’. I+ O+ Z+ Y+ / F’ I+ O+ Z+ Y+ I- O+ Z+ Y+ / F’ I+ O+ Z+ Y+ I- O+ Z+ Y+ I+ Oc Z+ Y+arrow_forward
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