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Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Textbook Question
Chapter 14, Problem 33P
The following hypothetical genotypes have genes A, B, and C corresponding to lacI,lacO, andlacZ but not necessarily in that order. Data in the table indicate whether
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The lac genotypes are as shown below:
P+OcZ-Y+A+// P¯O+Z+Y+A+
(i)
The lac operon consists of three structural genes, lacZ, lacY and lacA. Which
structural genes are involved in lactose metabolism? Explain.
(ii)
Draw and explain how lactose repress the gene expression in lac IS/I- heterozygote.
(iii)
What is the function of the promoter in the bacterial operon?
After irradiating wild-type cells of Neurospora (a haploid fungus), a geneticist finds two leucine-requiringauxotrophic mutants. He combines the two mutants ina heterokaryon and discovers that the heterokaryon isprototrophic.a. Were the mutations in the two auxotrophs in the samegene in the pathway for synthesizing leucine or in twodifferent genes in that pathway? Explain.b. Write the genotype of the two strains according toyour model.c. What progeny and in what proportions would youpredict from crossing the two auxotrophic mutants?(Assume independent assortment.)
For the genotypes and conditions (lactose present or absent) shown in the following Table 2,
predict whether the expression of beta-galactosidase is inducible (I), noninducible (N) or
constitutive (C). Explain your reason.
Table 2
Genotype
I*p*o°z+
Condition
(i)
No lactose
(ii)
(iii)
(iv)
I*P*O*Z+
I*P•O*Z*
I$P*O°Z*
Lactose
Lactose
Lactose
(v)
IP*0°Z+
No lactose
Chapter 14 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 14 - 12.1 Bacterial genomes frequently contain groups...Ch. 14 - Transcriptional regulation of operon gene...Ch. 14 - Why is it essential that bacterial cells be able...Ch. 14 - Identify similarities and differences between an...Ch. 14 - The transcription of -galactosidase and permease...Ch. 14 - 12.6 Is attenuation the product of an allosteric...Ch. 14 - The trpL region contains four repeated DNA...Ch. 14 - The CAP binding site in the lac promoter is the...Ch. 14 - What role does cAMP play in transcription of lac...Ch. 14 - How would a cap- mutation that produces an...
Ch. 14 - Explain the circumstances under which attenuation...Ch. 14 - Consider the transcription of genes of the...Ch. 14 - Describe the lytic and lysogenic life cycles of ...Ch. 14 - 12.14 Define antisense RNA, and describe how it...Ch. 14 - 12.15 Attenuation of trp operon transcription is...Ch. 14 - 12.16 In the lac operon, what are the likely...Ch. 14 - Identify which of the following lac operon haploid...Ch. 14 - Prob. 18PCh. 14 - 12.19 List possible genotypes for lac operon...Ch. 14 - Suppose each of the genotypes you listed in parts...Ch. 14 - 12.21 Four independent mutants (mutants A to D)...Ch. 14 - Suppose the lac operon partial diploid...Ch. 14 - Prob. 23PCh. 14 - 12.24 A repressible operon system, like the trp...Ch. 14 - 12.25 What is the likely effect of each of the...Ch. 14 - 12.26 Suppose that base substitution mutations...Ch. 14 - 12.27 Two different mutations affect. Mutant...Ch. 14 - How would mutations that inactivate each of the...Ch. 14 - The bacterial insertion sequence IS 10 uses...Ch. 14 - 12.34 Northern blot analysis is performed on...Ch. 14 - 12.37 The electrophoresis gel shown in part (a) is...Ch. 14 - Prob. 32PCh. 14 - The following hypothetical genotypes have genes A,...Ch. 14 - For an E. coli strain with the lac operongenotype...
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