Genetic Analysis: An Integrated Approach (2nd Edition)
Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 14, Problem 33P

The following hypothetical genotypes have genes A, B, and C corresponding to lacI,lacO, andlacZ but not necessarily in that order. Data in the table indicate whether β- galactosidase isproduced in the presence and absence of the inducer for each genotype. Use this data to identify the correspondence between A, B, and C and thelacI, lacO, and lacZ genes. Carefully explain your reasoning for identifying each gene.

Chapter 14, Problem 33P, The following hypothetical genotypes have genes A, B, and C corresponding to lacI,lacO, andlacZ but

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The lac genotypes are as shown below: P+OcZ-Y+A+// P¯O+Z+Y+A+ (i) The lac operon consists of three structural genes, lacZ, lacY and lacA. Which structural genes are involved in lactose metabolism? Explain. (ii) Draw and explain how lactose repress the gene expression in lac IS/I- heterozygote. (iii) What is the function of the promoter in the bacterial operon?
After irradiating wild-type cells of Neurospora (a haploid fungus), a geneticist finds two leucine-requiringauxotrophic mutants. He combines the two mutants ina heterokaryon and discovers that the heterokaryon isprototrophic.a. Were the mutations in the two auxotrophs in the samegene in the pathway for synthesizing leucine or in twodifferent genes in that pathway? Explain.b. Write the genotype of the two strains according toyour model.c. What progeny and in what proportions would youpredict from crossing the two auxotrophic mutants?(Assume independent assortment.)
For the genotypes and conditions (lactose present or absent) shown in the following Table 2, predict whether the expression of beta-galactosidase is inducible (I), noninducible (N) or constitutive (C). Explain your reason. Table 2 Genotype I*p*o°z+ Condition (i) No lactose (ii) (iii) (iv) I*P*O*Z+ I*P•O*Z* I$P*O°Z* Lactose Lactose Lactose (v) IP*0°Z+ No lactose

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Genetic Analysis: An Integrated Approach (2nd Edition)

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