Genetic Analysis: An Integrated Approach (2nd Edition)
Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 14, Problem 21P

Four independent lac - mutants (mutants A to D) are isolated in haploid strains of E. coli.

The strains have the following phenotypic characteristics:

Mutant A is lac-, but transcription of operon genes is induced by lactose.

Mutant B is lac- and has uninducible transcription of operon genes.

Mutant C is lac+ and has constitutive transcription of operon genes.

Mutant D is lac+ and has constitutive transcription of operon genes.

A microbiologist develops donor and recipient varieties of each mutant strain and crosses them with the results shown below. The table indicates whether inducible, constitutive, or noninducible transcription occurs, along with lac+ and lac- growth habit for each partial diploid. Assume each strain has a single mutation.

Chapter 14, Problem 21P, 12.21 Four independent mutants (mutants A to D) are isolated in haploid strains of E. coli.
The

Use the information to identify which lac operon gene is mutated in each strain.

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For each of the E. coli strains containing lac operon alleles listed, indicate whether the strain is inducible, constitutive, or unable to express beta-galactosidase and permease. (P+ and P- are functional and nonfunctional promoters, respectively) I+ P+ o+ Z- Y+ / I+ P+ oc Z+ Y+ I+ P+ o+ Z+ Y+ / I- P+ oc Z+ Y- I+ P+ o+ Z- Y+ / I- P+ oc Z+ Y- I- P- o+ Z+ Y- / I+ P+ oc Z- Y+ IS P+ o+ Z+ Y+ / I- P+ o+ Z+ Y-
A number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the highest beta-galactosidase enzyme activity, when grown in the lactose medium. O CAP+ r* p* o* z O CAP* I P* o* z* O CAP* r* P O* z* O CAP I P* O z*
A strain of E. coli has the genotypes shown below at the lac operon, where I = regulator gene, P = promoter, O = operator, Z = βgalactosidase gene, and Y = permease gene. The superscript+ indicates a wild-type allele,c indicates a constitutive mutation, and− indicates a defective mutation. For each genotype, indicate whether the enzyme will be synthesized or not synthesized when lactose is present or absent by placing a + for synthesis occurring and a − for synthesis not occurring in the appropriate blank. Explain your reasoning for each answer.

Chapter 14 Solutions

Genetic Analysis: An Integrated Approach (2nd Edition)

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