Genetic Analysis: An Integrated Approach (2nd Edition)
Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 14, Problem 18P
Summary Introduction

To analyze:

In the given table, the partial diploid genotypes are tested for the following aspescts:

Complete the following table for production of β galactosidase and permease in the presence or absence of lactose. Use (+) and (-) to indicate the presence of active enzyme and absence of active enzyme respectively. Determine the strain of partial diploid in lac + or lac -.

Genetic Analysis: An Integrated Approach (2nd Edition), Chapter 14, Problem 18P

Introduction:

In the lac operon, the partial diploids are produced by conjugation between F'× F; it will have two copies of the part of genome, hence, they are partially diploid. The lac operon partial diploids contain one copy of the lac operon information in the recipient bacterial chromosome and a second copy of it picked up by the F' plasmid. The genotypes of the partial diploids are written in a fashion that on left side, the F' segment is written, and on the right side recipient chromosome is written, and these are separated and indicated by (/).

Based on the studies of structural gene mutations, Jacob, Monod, and Colleagues concluded that lac + and lac - requires two protein-producing genes i.e. lacZ and lacY.

These two wild type alleles are always dominant to the mutant type alleles.

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For E. coli strains with the lac genotypes show below, use a plus sign (+) to indicate the synthesis of β-galactosidase and permease and a minus sign (–) to indicate no synthesis of the proteins.
For each of the following genotypes, explain how mutation (identified by a (-) will affect the organism grown in lactose medium. Indicate whether a) B-galactosidase will be synthesized or not, b) synthesis of B-galactosidase is inducible (1) or constitutive (C) and c) growth of the organism will occur or not. i. I+P+0-Z+Y+A+/l+P+O+Z-Y-A- ii. I-P+0+Z+Y+A+/I+P-O+Z+Y+A+
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Chapter 14 Solutions

Genetic Analysis: An Integrated Approach (2nd Edition)

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