Genetic Analysis: An Integrated Approach (2nd Edition)
Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 14, Problem 23P
Summary Introduction

To analyze:

An inducible operon of bacteria has three genes R, T, and S – same as lactose operon. These genes play a role in the regulation of transcription. These genes can be operator gene, regulatory gene, and structural gene (codes for structural enzyme). In the question, a table is provided in which "+" represents that the synthesis of structural enzyme is active and "-" represents no synthesis of structural enzyme. By using the given information, the question asked to determine from given genotype which gene is operator gene, which one is regulatory gene, and which one is structural gene.

Introduction:

A unit of DNA that has a group of genes controlled by a single promoter is termed as an operon. The operon possesses different regions (operator, promoter and structural genes); each region has a different role. Operator region is a short region of operon that controls transcription of genes by interacting with regulatory proteins. Regulatory proteins include proteins that act as transcription factor and facilitate the synthesis of protein in bacteria. Any protein which has its impact on the region of operon (DNA), which is transcribed in the transcription, is a regulatory protein.

The gene which codes for the protein other than the regulatory protein is termed as the structural gene. The product of structural genes is structural protein and enzymes.

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A number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the highest beta-galactosidase enzyme activity, when grown in the lactose medium. O CAP+ r* p* o* z O CAP* I P* o* z* O CAP* r* P O* z* O CAP I P* O z*
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The streptolysin S toxin made by S. pyogenes is encoded by a 9-gene operon, sagABCDEFGHI. Thinking about what a 3-line diagram would look like for this operon, answer the following questions. Write numeric answers only. For example, if your answer is 6 promoters, write only 6. 1) How many promoters control the expression of these genes? 2) How many locations does RNA Polymerase bind to get full expression of these genes? 3) How many ribosome binding sites are needed for full protein expression? 4) How many start codons will be needed for full protein expression? 5) How many mRNA strands will be produced with full operon expression? 6) How many proteins will be produced with full protein expression? 1

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Genetic Analysis: An Integrated Approach (2nd Edition)

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