Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 14, Problem 8P
The CAP binding site in the lac promoter is the location of positive regulation of gene expression for the operon. Identify what binds at this site to produce positive regulation, under what circumstances binding occurs, and how binding exerts a positive effect.
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Describe the mechanism by which cAMP receptorprotein (CRP), the regulatory protein for catabolite repression, functions. Use the lactose operon as an example.
Explain why inactivation of the O1 sequence of the lac operator almost completely abolishes repression of the lac operon.
Mutation in the regulatory gene in the lac operon results in the formation of an inactive repressor. List and explain the effect of such a mutation on the expression of the structural gene for lactose in E.coli?
Chapter 14 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 14 - 12.1 Bacterial genomes frequently contain groups...Ch. 14 - Transcriptional regulation of operon gene...Ch. 14 - Why is it essential that bacterial cells be able...Ch. 14 - Identify similarities and differences between an...Ch. 14 - The transcription of -galactosidase and permease...Ch. 14 - 12.6 Is attenuation the product of an allosteric...Ch. 14 - The trpL region contains four repeated DNA...Ch. 14 - The CAP binding site in the lac promoter is the...Ch. 14 - What role does cAMP play in transcription of lac...Ch. 14 - How would a cap- mutation that produces an...
Ch. 14 - Explain the circumstances under which attenuation...Ch. 14 - Consider the transcription of genes of the...Ch. 14 - Describe the lytic and lysogenic life cycles of ...Ch. 14 - 12.14 Define antisense RNA, and describe how it...Ch. 14 - 12.15 Attenuation of trp operon transcription is...Ch. 14 - 12.16 In the lac operon, what are the likely...Ch. 14 - Identify which of the following lac operon haploid...Ch. 14 - Prob. 18PCh. 14 - 12.19 List possible genotypes for lac operon...Ch. 14 - Suppose each of the genotypes you listed in parts...Ch. 14 - 12.21 Four independent mutants (mutants A to D)...Ch. 14 - Suppose the lac operon partial diploid...Ch. 14 - Prob. 23PCh. 14 - 12.24 A repressible operon system, like the trp...Ch. 14 - 12.25 What is the likely effect of each of the...Ch. 14 - 12.26 Suppose that base substitution mutations...Ch. 14 - 12.27 Two different mutations affect. Mutant...Ch. 14 - How would mutations that inactivate each of the...Ch. 14 - The bacterial insertion sequence IS 10 uses...Ch. 14 - 12.34 Northern blot analysis is performed on...Ch. 14 - 12.37 The electrophoresis gel shown in part (a) is...Ch. 14 - Prob. 32PCh. 14 - The following hypothetical genotypes have genes A,...Ch. 14 - For an E. coli strain with the lac operongenotype...
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- For the following types of transcriptional control, indicate whether the protein produced by the regulator gene will be synthesized initially as an active repressor or as an inactive repressor. Q. Negative control in a repressible operonarrow_forwardDescribe the role of the CRP–cAMP complex in positiveregulation of the lactose operon in E. coli.arrow_forwardOne way that regulation is often studied is by examining the effects of mutations. For example, mutations within the parts of the lac operon have been well studied. Which of these mutants would be considered a constitutive mutant (the operon is always on)? a mutation in CAP that prevents the binding of CAMP a mutation in one of the structural genes that prevents the transport of lactose into the cell a mutation that disables the promoter for the operon a mutation that disables the repressorarrow_forward
- For the following types of transcriptional control, indicate whether the protein produced by the regulator gene will be synthesized initially as an active repressor or as an inactive repressor. Q. Negative control in an inducible operonarrow_forwardWhen referring to attenuation in the regulation of the trp operon it would be safe to say that, when there are high levels of tryptophan available to the organism, transcription termination at the attenuator region is likely the ribosomes stall during translation of the attenuator region translation termination of the trp operon is likely the trp operon is transcribed at relatively high levels tryptophan inactivates the repressor proteinarrow_forwardWhen CAMP binds to the Catabolite Activator Protein (CAP), it causes a conformational change on the protein, which results in binding to the CAP- binding site on the DNA and molecule is known as a(n) of the lac operon. This conformational change induced by the binding of another transcription activation; allosteric effect transcription repression; binding effect translation; distortion effect mutation; interactive effect attenuation; compression effect O O O Oarrow_forward
- Supply the words “off" or “on" in the blanks below: In an inducible operon, transcription is normally and must be turned In a repressible operon, transcription is normally and must be turned Briefly describe the overall function of the structural genes in the lac operon. Briefly describe the overall function of the structural genes in the trp operon. For the following descriptions of the bacterial lac operon, determine if lactose is present (P) or absent (A). The repressor is bound to the operator. The repressor is inactive. RNA polymerase transcribes the structural genes. Allolactose is bound to the repressor For the following descriptions of the bacterial trp operon, determine if tryptophan levels are high (H) or low (L). The repressor is bound to the operator. The repressor is inactive. RNA polymerase transcribes the structural genes. Tryptophan is bound to the repressor.arrow_forwardUnder what conditions would you expect to see high levels of expression of the structural genes in the lac operon? Group of answer choices Lactose is absent and glucose is absent Lactose is absent and glucose is present Lactose is present and glucose is present None of these Lactose is present and glucose is absentarrow_forwardIn the lac operon system, do the inducers act at the transcription or translation level? Explain why it acts in that level in a short but concise way.arrow_forward
- In terms of the efficient use of bacterial resources, explain why it is necessary for lac operon expression to be controlled both by the lac repressor and by a cyclic AMP receptor protein rather than just by the lac repressor alone.arrow_forwardYou have isolated two different mutants (reg1 and reg2) causing constitutive expression of the emu operon (emu1 emu2). One mutant contains a defect in a DNA-binding site, and the other has a loss-of-function defect in the gene encoding a protein that binds to the site. Is the DNA-binding protein a positive or negative regulator of gene expression? Explain. To determine which mutant has a defect in the site and which one has a mutation in the binding protein, you decide to do an analysis using F′ plasmids. Assuming you can assay levels of the Emu1 and Emu2 proteins, what results do you predict for the two strains (i and ii; see descriptions below) if reg2 encodes the regulatory protein and reg1 is the regulatory site? Explain. F′ (reg1− reg2+ emu1− emu2+)/reg1+ reg2+ emu1+ emu2− F′ (reg1+ reg2− emu1− emu2+)/reg1+ reg2+ emu1+ emu2−arrow_forwardTryptophan (Trp) attenuation is an extra mechanism of regulation of trp operon when trp level is too high. Attenuation causes premature transcription termination. c) Review the region that is responsible for trp attenuation during low trp level.arrow_forward
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