Use the diagram below to answer the following question. Identifythe protein that was run on Mass Spec?(HINT: You will need to look up the molecular weights)Relative Abundance, %800Human IgGCaseinESI MSPepsin18+ 15+16+1200Cyanogen bromide14+-13+m/z12+11+160023,984 +/-210+1400 1410 14209+24,024 +/-2200024,093+/-28+

We are given the spectrogram of an ESI-MS (Electrospray ionization Mass Spectroscopy). In this…

Answered: Use the diagram below to answer the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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One OD unit of A260 is 50 ug/ml dsDNA. You have 2.75 ml genomic DNA (stock). You took 50 ul of the DNA sample and mixed it with 950 ul water and measured the A260 absorption. The diluted sample absorbed 0.14 units of A260. The DNA concentration of the stock is ug/ml, considering the dilution factor of (Note: ug microgram, ul- microliter). 70/10 140/20 280/50 O 560/100
You put 50 uL of 0.1 mg of protein into a 1 cm pathlength cuvette containing 3 mL of LDH reaction mixture and measure a slope of 0.125 abs/min in an LDH assay. What is the reaction rate in uM/min? Show your work.
What is the essence of conducting protein extraction procedures in the field of Biology Note: Provide an answer with 100-200 words
What is the best interpretation for the results below? Test/s Results Ninhydrin + Fohl's Test Biuret Test Sakaguchi O The unknown sample is a dipeptide that contains arginine and cysteine O The unknown sample is a dipeptide that contains arginine and cystine O The unknown sample is a free amino acid that contains arginine and cysteine O The unknown sample is a free amino acid that contains tyrosine and cystine as residues
Answer the following questions about monosaccharide B. Draw the β anomer of B in a Haworth projection.
The following protein quantification utilizes Cu ions except fora. Lowryb. Biuretc.Bradford
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. b= 0.24 Incorrect 0.00356 incorrect RNA (µg) 0.00 11.26 22.52 33.78 45.04 Absorbance at 260 nm 0.240 0.566 0.863 1.217 1.516 #g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of t 0.828.
Look for 5 color reaction/test that can be used to identify amino acids. Indicate also the color for positive results and what causes this results .
Use the information on the proteins given to solve the questions: A. Draw a UV-Vis spectrum of each protein (A, B, C, and D) from 240 – 480 nm if they were all present at 20 µM. B. How can you create a two-step purification procedure that could be used to purify protein A from the other proteins. In addition, include a chromotagram as well.
When you graph your protein assay data in Excel, should you include the absorbance of your unknown BSA sample? Explain why or why not
3 app.formative.com/formatives/63763915dfee3ba940357e25 4 5 6 7 8 9 10 11 Type here to search Sample 1 | | |||||||||||||||| || || | ||||||| ||| |||||||| Sample 2 19. An individual found with blood on her clothing was accused of a crime. DNA fingerprinting of the following samples was carried out: Sample 3 Sample 1: Blood of the accused individual Sample 2: Blood of the victim Sample 3: Blood sample found at the crime scene Sample 4: DNA evidence found on the accused individual's clothing Sample 5: DNA evidence found in the accused individual's car |||||||||||||||||| | || | Sample 4 12 13 14 Sample 5 O 15 16 17 18 19 20 21 * Is there DNA evidence to support the arrest of the accused individual for this crime? Q B fa
In SIze Exclusion Chromatography, how does the instrument works that it can give data for both the identity and concentration of the high molecular protein, specifically explaining each part of the instrument and on where separation takes place, and the suitable detection method that can be used.

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