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Biochemistry
Protein Analysis.

Protein Analysis.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Protein Analysis. A student is studying the protein content of "sigarilyas" (Psophocarpus tetragonolobus) by Biuret Assay. Tables 1 and 2 show the calibration and sample analysis data respectively. Fill-out the missing data in the tables, construct the calibration plot using MS Excel, and determine the over-all protein concentration of the samples. Submit the PDF copy of your output. Table 1. Calibration Data Standard Vol of BSA (ml)* Equiv. Conc. (mg/ml)** Absorbance at 550 nm 0.10 0.062 2 0.20 0.115 3 0.40 0.212 0.80 0.436 1.60 *BSA means bovine serum albumin (15 mg/ml) 5 0.892 ** Final dilution volume is 2.5 ml Table 2. Sample Analysis Absorbance at Diluted Volume of Dilution Over-all Protein Conc. 550 nm 0.145 Sample Volume (ml) Dilution (ml) Factor (mg/ml) 1. 0.50 2.0 2 0.222 0.30 3.0 3 0.679 0.50 4.0 4 0.421 0.30 1.5 0.738 0.50 2.5 MS Excel Calibration Plot
Transcribed Image Text:Protein Analysis. A student is studying the protein content of "sigarilyas" (Psophocarpus tetragonolobus) by Biuret Assay. Tables 1 and 2 show the calibration and sample analysis data respectively. Fill-out the missing data in the tables, construct the calibration plot using MS Excel, and determine the over-all protein concentration of the samples. Submit the PDF copy of your output. Table 1. Calibration Data Standard Vol of BSA (ml)* Equiv. Conc. (mg/ml)** Absorbance at 550 nm 0.10 0.062 2 0.20 0.115 3 0.40 0.212 0.80 0.436 1.60 *BSA means bovine serum albumin (15 mg/ml) 5 0.892 ** Final dilution volume is 2.5 ml Table 2. Sample Analysis Absorbance at Diluted Volume of Dilution Over-all Protein Conc. 550 nm 0.145 Sample Volume (ml) Dilution (ml) Factor (mg/ml) 1. 0.50 2.0 2 0.222 0.30 3.0 3 0.679 0.50 4.0 4 0.421 0.30 1.5 0.738 0.50 2.5 MS Excel Calibration Plot
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