Protein Analysis.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
complete the table below

Transcribed Image Text:Protein Analysis. A student is studying the protein content of "sigarilyas" (Psophocarpus tetragonolobus) by Biuret
Assay. Tables 1 and 2 show the calibration and sample analysis data respectively. Fill-out the missing data in the
tables, construct the calibration plot using MS Excel, and determine the over-all protein concentration of the
samples. Submit the PDF copy of your output.
Table 1. Calibration Data
Standard
Vol of BSA (ml)*
Equiv. Conc. (mg/ml)**
Absorbance at 550 nm
0.10
0.062
2
0.20
0.115
3
0.40
0.212
0.80
0.436
1.60
*BSA means bovine serum albumin (15 mg/ml)
5
0.892
** Final dilution volume is 2.5 ml
Table 2. Sample Analysis
Absorbance at
Diluted
Volume of
Dilution
Over-all Protein Conc.
550 nm
0.145
Sample
Volume (ml)
Dilution (ml)
Factor
(mg/ml)
1.
0.50
2.0
2
0.222
0.30
3.0
3
0.679
0.50
4.0
4
0.421
0.30
1.5
0.738
0.50
2.5
MS Excel Calibration Plot
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