KDa 97.4 66.24 45.0 31.0 21.5 14.4 8-1 1-d s-2 For condition 1 - For condition 2 - For condition 3 - For condition 4 - For condition 5 - P-2 S-3 E-d s-4 t-d S-5 S-d a For each condition (there are 5 different conditions), is there a higher percentage of the total protein in the supernatant, pellet, or is it about equal?
KDa 97.4 66.24 45.0 31.0 21.5 14.4 8-1 1-d s-2 For condition 1 - For condition 2 - For condition 3 - For condition 4 - For condition 5 - P-2 S-3 E-d s-4 t-d S-5 S-d a For each condition (there are 5 different conditions), is there a higher percentage of the total protein in the supernatant, pellet, or is it about equal?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:### Protein Analysis for Different Conditions
#### Gel Electrophoresis Image Analysis
The image depicts a gel electrophoresis result. The lanes are labeled as follows:
- **s-1, p-1**: Supernatant and pellet for Condition 1
- **s-2, p-2**: Supernatant and pellet for Condition 2
- **s-3, p-3**: Supernatant and pellet for Condition 3
- **s-4, p-4**: Supernatant and pellet for Condition 4
- **s-5, p-5**: Supernatant and pellet for Condition 5
The left-hand side indicates molecular weight markers, labeled in kDa (kilodaltons):
- 97.4, 66.2, 45.0, 31.0, 21.5, 14.4
#### Question for Analysis
For each condition, determine whether there is a higher percentage of the total protein in:
- The supernatant
- The pellet
- Or if it is about equal
**For condition 1 -**
**For condition 2 -**
**For condition 3 -**
**For condition 4 -**
**For condition 5 -**
Use this information to understand protein separation and localization under various experimental conditions.
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