RNA Transcription, Translation, and Mutation Worksheet First, here is a strand of DNA, This strand contains both a gene and its promoter region. Circle the promoter region in blue, draw a yellow box around the TATA box, draw a green box around the start codon, and draw a red box around the stop codon: TATATATATTACGTTGCATACGCTCAACGGTCGAAACTGCATGGGCAC ATATATATAATGCAACGTATGCGAGTTGCCAGCTTTGACGTACCCG Now imagine this gene has been transcribed into RNA. What would that RNA strand look like? Before the above RNA strand can be translated, a few modifications must first take place (in eukaryotes). What are they? 1) 2) 3) Using a codon chart of your choice (one can be found here, or here) translate the above RNA transcript (assume no splicing took place). Write the three letter abbreviations for the amino acids in the image below: OTOR Now imagine that a mutation took place in the original strand of DNA (marked in red) TATATATATTACGTTOCATACCCTCAACGGTCGAAACTGCATG ATATATATAATGCAACCTATO GGAGTTecCAGCTTTGACGT What type of mutation was this? What kind of effect did it have on the polypeptide?

Human Anatomy & Physiology (11th Edition)
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RNA Transcription, Translation, and Mutation
Worksheet
First, here is a strand of DNA. This strand contains both a gene and its promoter region. Circle
the promoter region in blue, draw a yellow box around the TATA box, draw a green box around
the start codon, and draw a red box around the stop codon:
TATATATATTACGTTGCATACGCTCAACGGTCGAAACTGCATGGGCAC
ATATATATAATGCAACGTATGCGAGTTGCCAGCTTTGACGTACCCG
Now imagine this gene has been transcribed into RNA. What would that RNA strand look like?
Before the above RNA strand can be translated, a few modifications must first take place (in
eukaryotes). What are they?
1)
2)
3)
Using a codon chart of your choice (one can be found here, or here) translate the above RNA
transcript (assume no splicing took place). Write the three letter abbreviations for the amino
acids in the image below:
Now imagine that a mutation took place in the original strand of DNA (marked in red)
TATATATATTACGTTGCATACCCTCAACGGTCGAAACTGCATG
ATATATATAATGCAACCTATccGAGTTecCAGCTTTGACGT
What type of mutation was this?
What kind of effect did it have on the polypeptide?
Transcribed Image Text:RNA Transcription, Translation, and Mutation Worksheet First, here is a strand of DNA. This strand contains both a gene and its promoter region. Circle the promoter region in blue, draw a yellow box around the TATA box, draw a green box around the start codon, and draw a red box around the stop codon: TATATATATTACGTTGCATACGCTCAACGGTCGAAACTGCATGGGCAC ATATATATAATGCAACGTATGCGAGTTGCCAGCTTTGACGTACCCG Now imagine this gene has been transcribed into RNA. What would that RNA strand look like? Before the above RNA strand can be translated, a few modifications must first take place (in eukaryotes). What are they? 1) 2) 3) Using a codon chart of your choice (one can be found here, or here) translate the above RNA transcript (assume no splicing took place). Write the three letter abbreviations for the amino acids in the image below: Now imagine that a mutation took place in the original strand of DNA (marked in red) TATATATATTACGTTGCATACCCTCAACGGTCGAAACTGCATG ATATATATAATGCAACCTATccGAGTTecCAGCTTTGACGT What type of mutation was this? What kind of effect did it have on the polypeptide?
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