The following sequence is a mutant version of the above gene that is present in a mutant bacterial strain. The nature of the mutation is that the base-pair bolded above in the wild-type sequence has been deleted. The sequence given is from the point where transcription starts to the point where transcription ends. 5'-АСТТCGАТАTGTСТААААТАСGATCGATCTGTGGGGCСТАGCТАGСТААССАGAGACGCTACCG-3' 3'-TGAAGCTАТАСAGATTTтТАTGСТАGСТAGACACCCCGGATCGATCGАТTGGTCTCTGCGATGGC-5. (e) Write out the amino acid sequence of any protein that is encoded by this mutated gene. Make sure to label the N and C termini of your molecule. (f) What is the nature of the mutation and how does it affect the protein?
The following sequence is a mutant version of the above gene that is present in a mutant bacterial strain. The nature of the mutation is that the base-pair bolded above in the wild-type sequence has been deleted. The sequence given is from the point where transcription starts to the point where transcription ends. 5'-АСТТCGАТАTGTСТААААТАСGATCGATCTGTGGGGCСТАGCТАGСТААССАGAGACGCTACCG-3' 3'-TGAAGCTАТАСAGATTTтТАTGСТАGСТAGACACCCCGGATCGATCGАТTGGTCTCTGCGATGGC-5. (e) Write out the amino acid sequence of any protein that is encoded by this mutated gene. Make sure to label the N and C termini of your molecule. (f) What is the nature of the mutation and how does it affect the protein?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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E and F
Transcribed Image Text:**9. The following sequence is a wild-type bacterial gene that encodes a short protein. The sequence given is from the point where transcription starts (called “+1”) to the point where transcription ends. Transcription goes left to right.**
5’-ACTTGAGTAATGATTGAATAAAACTGACGTAGTCAGGTCCGACGGTGCGCATCACCCGAGGGCTACGTAAGTCACAGCAGCGGTACTCCG-3’
3’-TGAACTCATTACTTATTATTTTGACTGCATCAGTCCAGGCTGCCACGCGTAGTGGGCTCCCGATGCATTCAGTCTGTCGCCATGGGC-5’
**(a) Which strand (the upper or the lower) is used as the template in transcription?**
**(b) Write out the first 20 bases of the RNA transcribed from this wild-type gene. Make sure to label the 5’ and 3’ ends of your molecule.**
**(c) Write out the amino acid sequence of any protein that is encoded by this wild-type gene. Make sure to label the N and C termini of your molecule.**
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**The following sequence is a mutant version of the above gene that is present in a mutant bacterial strain. The nature of the mutation is that the base-pair bolded above in the wild-type sequence has been deleted. The sequence given is from the point where transcription starts to the point where transcription ends.**
5’-ACTTGAGTAATGATTGAATAAAACTGACGTAGTCAGGTCCGACGGTGCGCATCACCCGAGGGCTACGTAAGTCACAGCAGCGGTACTCC -3’
3’-TGAACTCATTACTTATTATTTTGACTGCATCAGTCCAGGCTGCCACGCGTAGTGGGCTCCCGATGCATTCAGTCTGTCGCCATGGGT-5’
**(e) Write out the amino acid sequence of any protein that is encoded by this mutated gene. Make sure to label the N and C termini of your molecule.**
**(f) What is the nature of the mutation and how does it affect the protein?**
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