Q5. A sh mutant called shXS is isolated that produces a truncated polypeptide of only 6 AAs in length. Describe a single basepair DNA change that would lead to this truncated version of the protein. Multiple options are possible (100 words max.) [10%] Q6. A single basepair mutant in the sh gene (shNH) was found to have an A mutated into a G at the 3rd position of the 3rd codon of the sh gene:   WILD-TYPE: 5’-tataatgggcttaacaATGAGTAAAAGAGGTCCTTTACTCCGGTATCACCAATAGaaatattatttaa-3’   Mutant shNH 5’-tataatgggcttaacaATGAGTAAGAGAGGTCCTTTACTCCGGTATCACCAATAGaaatattatttaa-3’   What are the functional consequences of this mutation for the Sh protein? Motivate your answer (100 words max.) [10%]

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