QUESTION 3 Imagine that you repeat the tRNA Selection experiment with modifications as follows: 1. Synthesize mRNA containing A's and G's only (poly-AG in random order). 2. Convert the amino acid Glutamic acid (Glu) on its tRNA to the amino acid Glutamine (Gln) as shown below. 3. Mix your poly-AG RNA, your artificial tRNA, and cell extract (contains ribosomes, amino acids, all normal tRNAs, and the energy source for translation). Phe Tyr 5' of of GAA GAA Glutamic acid (Glu) is encoded by GAA and GAG, while Glutamine (Gln) is encoded only by CAA and CAG. What does the outcome of this experiment tell us about translation? O Multiple codons code for each amino acid. An amino acid is selected based on the identity of the tRNA. The ribosome does the translation, i.e. it selects the amino acid regardless of the identify of the tRNA. The ribosome reads mRNA 3 bases at a time.

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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QUESTION 3
Imagine that you repeat the tRNA Selection experiment with modifications as follows:
1. Synthesize mRNA containing A's and G's only (poly-AG in random order).
2. Convert the amino acid Glutamic acid (Glu) on its tRNA to the amino acid Glutamine (Gln) as shown below.
3. Mix your poly-AG RNA, your artificial tRNA, and cell extract (contains ribosomes, amino acids, all normal tRNAs, and the energy source
for translation).
Phe
Tyr
5'
of of
GAA
GAA
Glutamic acid (Glu) is encoded by GAA and GAG, while Glutamine (Gln) is encoded only by CAA and CAG.
What does the outcome of this experiment tell us about translation?
O Multiple codons code for each amino acid.
An amino acid is selected based on the identity of the tRNA.
O The ribosome does the translation, i.e. it selects the amino acid regardless of the identify of the tRNA.
O The ribosome reads mRNA 3 bases at a time.
Transcribed Image Text:QUESTION 3 Imagine that you repeat the tRNA Selection experiment with modifications as follows: 1. Synthesize mRNA containing A's and G's only (poly-AG in random order). 2. Convert the amino acid Glutamic acid (Glu) on its tRNA to the amino acid Glutamine (Gln) as shown below. 3. Mix your poly-AG RNA, your artificial tRNA, and cell extract (contains ribosomes, amino acids, all normal tRNAs, and the energy source for translation). Phe Tyr 5' of of GAA GAA Glutamic acid (Glu) is encoded by GAA and GAG, while Glutamine (Gln) is encoded only by CAA and CAG. What does the outcome of this experiment tell us about translation? O Multiple codons code for each amino acid. An amino acid is selected based on the identity of the tRNA. O The ribosome does the translation, i.e. it selects the amino acid regardless of the identify of the tRNA. O The ribosome reads mRNA 3 bases at a time.
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