PROBLEMS 8.1 An enzyme-catalysed reaction was found to be affected by two inhibitors A and B. The following results were obtained at fixed total enzyme concentration: Substrate conc" Initial velocity (absorbance units per minute) (mmol l-) Uninhibited With 1 mmol I-A With 1 mmol I-B 50 20 10 0.684 1.08 1.43 1.02 0.798 0.657 0.549 0.653 0.468 0.363 1.01 3.3 2.5 2.0 0.649 0.476 0.374 0.311 0.296 0.250 Comment on these results.
PROBLEMS 8.1 An enzyme-catalysed reaction was found to be affected by two inhibitors A and B. The following results were obtained at fixed total enzyme concentration: Substrate conc" Initial velocity (absorbance units per minute) (mmol l-) Uninhibited With 1 mmol I-A With 1 mmol I-B 50 20 10 0.684 1.08 1.43 1.02 0.798 0.657 0.549 0.653 0.468 0.363 1.01 3.3 2.5 2.0 0.649 0.476 0.374 0.311 0.296 0.250 Comment on these results.
Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Transcribed Image Text:PROBLEMS
8.1 An enzyme-catalysed reaction was found to be affected by two inhibitors A and
B. The following results were obtained at fixed total enzyme čoncentration:
Substrate conc"
Initial velocity (absorbance units per minute)
(mmol l-)
With 1 mmol I-B
Uninhibited
With 1 mmol l- A
0.684
50
20
1.08
0.653
0.468
10
1.43
1.01
0.649
0.476
0.374
0.311
5
1.02
0.363
0.798
0.657
3.3
0.296
2.5
0.250
2.0
0.549
Comment on these results.
8.2 The system investigated in problem 7.1 was investigated again under identical
conditions but in the presence of an inhibitor, giving the following data:
40.0
6.67 10.0
156
20.0
Substrate conc" (mmol 1-1)
Initial velocity (umol 1- min-1)
5.0
100 122
222
278
Determine the type of inhibition. If K, for this system is 2.9 mmol 1-', calculate the
inhibitor concenträtion present.
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