How many proteins are possible in a gene with 4 exons?• Label the exons A, B, C, and D.• Draw out the possibilities• Hint: list the singletons, the doubles, the triples, etc General formula: 2n-1• Why the -1? And how can you find that without using the general formula
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How many proteins are possible in a gene with 4 exons?
• Label the exons A, B, C, and D.
• Draw out the possibilities
• Hint: list the singletons, the doubles, the triples, etc
General formula: 2n-1
• Why the -1?
And how can you find that without using the general formula
Step by step
Solved in 2 steps
- In the following gel showing stained bands of the Alu insertion sequence, what is the genotype of individual 2? 941 bp 641 bp->>> 1 2 3 4 5 6 Homozygous for the 641 bp sequence that does not contain in the Alu insertion Heterozygous, containing one 941 bp sequence and one 641 bp sequence O Homozygous for the 941 bp sequence containing the Alu insertionYou have the following DNA coding sequence of a wild-type allele: 5’-ATG TTC CAG CTA GAT GAT ATG CTG GTA ATT GGG GAA CGC GCG CGG TAA-3’ 1. For the second, third, fourth, and fifth codons, write all possible anticodon sequences (left-to-right, 5’- 3’), including anticodons with wobble and inosine.2ith the diagram provided please write the polypeptide. sequence obtained from the translation of the black allele gene
- • The image shows the sequence of tandem repetitive DNA (highlighted in green) at one locus in the genome, with the copy of the locus on each chromosome within the homologous pair shown (and listed as #1 and #2). Answer the following questions. 。 What type of repetitive DNA is shown? [Select] • You want to do a PCR of this locus. If one primer's sequence is 5'-TCGAGGTTC-3', then what is the second primer sequence needed to accurately PCR the locus? Choose a primer of 9 bases long. [Select] 。 You follow the PCR with an agarose gel electrophoresis. When preparing for agarose gel electrophoresis, which electrode should be placed nearest the starting point of the sample (the product of the PCR)? [Select] 。 Which allele would travel less far in your gel? [Select] #1: 5'-TCGAGGTTCCAACAACAACAACAACAACAACAACAACAAGGATCTACT-3' 3'-AGCTCCAAGGTTGTTGTTGTTGTTGTTGTTGTTGTTGTTCCTAGATGA-5' #2: 5'-TCGAGGTTCCAACAACAACAACAACAACAACAACAACAACAACAACAACAAGGATCTACT-3'…A portion of the coding sequence of a cloned gene is shown here:5΄–GCCCCCGATCTACATCATTACGGCGAT–3΄3΄–CGGGGGCTAGATGTAGTAATGCCGCTA–5΄This portion of the gene encodes a polypeptide with the aminoacid sequence alanine–proline–aspartic acid–leucine–histidine–histidine–tyrosine–glycine–aspartic acid. Using the method ofsite- directed mutagenesis, a researcher wants to change the leucinecodon into an arginine codon, using an oligonucleotide that is19 nucleotides long. What is the sequence of the oligonucleotidethat should be used? Designate the 5′ and 3′ ends of the oligonucleotidein your answer. Note: The mismatch should be in the middleof the oligonucleotide, and a 1-base mismatch is preferableover a 2- or 3-base mismatch. Use the bottom strand as the templatestrand for this site-directed mutagenesis experiment.2. Consider the following DNA molecule. Assume this is the DNA sequence of the entire chromosomes. Assume there are no introns. 3' AATTAGCAGATGCATGATGCAATTACTAGCATGTAAGTA 5' 5' TПААТСGTТСТАСGTACTАCGTTAATGATCGTACATTCAT 3' Based on your knowledge of open reading frames, list the amino acid sequences of the possible protein or proteins that could be produced from this DNA sequence. You may use any codon table of your liking to complete the work. a. b. Suppose there was a sister chromatid generated from the chromosome sequence shown above. What would be its DNA sequence (provide the sequence in the space below. Be sure to identify the 5' and 3'ends)?
- Examine the following mRNA transcripts: Wild type: 5' CACUGAUGCACGGAUCAU 3' Mutant: 5' CACUGAUGCACGGAUGAU 3' What kind of mutation is occurring in the mutant sequence compared to the wild type sequence? SECOND BASE Phenylalanine (Phe) UCU UAU Tyrosine (Tyr) UGU Cysteine (Cys) UUC UCc UAC UGC Serine (Ser) UAA -Stop codon UAG -Stop codon UGA -Stop codon uGa -Tryptophan (Trp) UUA UCA Leucine (Leu) UUG uca CUU CCU Cuc CAU CAC Histidine (His) CGU Leucine (Leu) Proline (Pro) C. CUA CuG Dainine (Arg) CCA cca CGA CAA CAG Glutamine (GIin) cGG AUU AUC ACU ACC ACA AAU AGU Isoleucine (lle) AAC Asparagine (Asn) AGC Serine (Ser) Threonine (Thr) AUA AAA Methionine (Met) Sta AGA AGG ACG AAG FLysine (Lys) Arginine (Arg) AUG - codon GUU Gcu Gcc GCA GAU GGU GUC GAc Aspartic acid (Asp) G. GUA Valine (Val) Alanine (Ala) Glycine (Gly) GAA GAG Glutamic acid (Glu) GGA GaG GUG aca O Silent O Missense O Nonsense O Frameshift FIRST BASE THIRD BASEplz explain with thorough explanation. In examining Figure 3-19, what do you think is the mainreason for the difference in size of yeast and humanmtDNA?
- Consider the following DNA sequence, which codes for a short polypeptide: 5'-ATGGGCTTAGCGTAGGTTAGT-3' Determine the mRNA transcript of this sequence. You have to write these sequences from the 5' end to the 3' end and indicate those ends as shown in the original sequence in order to get the full mark. How many amino acids will make up this polypeptide? Determine the first four anticodons that will be used in order to translate this sequence.The human RefSeq of the entire first exon of a geneinvolved in Brugada syndrome (a cardiac disordercharacterized by an abnormal electrocardiogram andan increased risk of sudden heart failure) is:5′ CAACGCTTAGGATGTGCGGAGCCT 3′The genomic DNA of four people (1–4), three ofwhom have the disorder, was subjected to singlemolecule sequencing. The following sequences represent all those obtained from each person. Nucleotidesdifferent from the RefSeq are underlined. Individual 1:5′ CAACGCTTAGGATGTGCGGAGCCT 3′and5′ CAACGCTTAGGATGTGCGGAGACT 3′Individual 2:5′ CAACGCTTAGGATGTGAGGAGCCT 3′Individual 3:5′ CAACGCTTAGGATGTGCGGAGCCT 3′and5′ CAACGCTTAGGATGGCGGAGCCT 3′Individual 4:5′ CAACGCTTAGGATGTGCGGAGCCT 3′and5′ CAACGCTTAGGATGTGTGGAGCCT 3′a. The first exon of the RefSeq copy of this gene includes the start codon. Write as much of the aminoacid sequence of the encoded protein as possible,indicating the N-to-C polarity.b. Are any of these individuals homozygotes? If so,which person and what allele?c. Is…If one strand of DNA has the sequence 5'-C-T-A-G-C-G-T-T-A-3', what sequence would appear opposite it on the other strand? Enter the complementary sequence of letters separated by hyphens. • View Available Hint(s) 3'- 3'-G-A-T-C-G-C-A-A-T-5' -5'