Give only typing answer with explanation and conclusion Information: 1_Green Fluorescent Protein 2_nucleotide sequence, Amino acid sequence, and primers are obtained. 3_PCR protocol already described 4_bp has been calculations and estimated agarose gel image already designed. Questions: How do you analyze whether your target protein is expressed by E. coli cells. Explain your analysis method in detail and give information about the results you expect (in detail please)

Q: Outline the major steps invalved in recombinant DNA technalogy required to clane a gene into a…

A: INTRODUCTION Recombinant DNA technology In recombinant DNA technology we insert our gene of interest…

Q: You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa 100 kDa -80 kDa 80 kDa…

A: you have asked multiple question please repost the question and mention the sub parts needed to be…

Q: For this activity you will draw out the first steps of the PCR reaction, using the following as a…

A: Introduction  PCR is the revolutionized technique invented by Karry Mullis in 1983, for which he got…

Q: What is the size of the PCR product (Hse gene) observed from the gel photo?

A: ** The expected size of PCR product is 357+280bp, or 637np.                                     **…

Q: Select all that would be true if I had a missense mutation in an gene: The missense mutant protein…

A: A missense mutation is a type of point mutation in which a single nucleotide  change results in a…

Q: E. coli bacterium Bacterial chromosome Plasmid Isolate plasmid O Recombinant DNA plasmid Copyright ©…

A: Introduction :- Gene cloning is the process of creating perfect duplicates of a gene or DNA sequence…

Q: Why does it make sense to look for ARGs and not antibiotic-resistant organisms themselves?

A: Antibiotic resistance genes (ARGs) are segments of DNA that encode for proteins or enzymes that…

Q: You complete a comparative transcriptomics experiment, where RNA from a plant sample grown in sandy…

A: The researchers compared the gene expression differences between plants cultivated in silty soil and…

Q: True or False: 1. The 6X HIS tag is required for a eukaryotic protein to be expressed in E. coli 2.…

A: Polyhistidine tag is added to a protein and helps in its purification. A recombinant protein can…

Q: ased on the knowledge you gained from the cloning module, which of the lanes in the urs is…

A: Agarose gel electrophoresis is used to separate DNA molecules based on their size and analyze DNA…

Q: Compare and contrast selective breeding with rDNA technology. Describe a minimum of three (3)…

A: Recombinant DNA technology or the genetic engineering method is used to manipulate the DNA according…

Q: BOTTOM The sequence depicted on the gel is 5'-GTGATGTAG-3' The sequence depicted on the gel is 3'-…

A: This method is used for DNA sequencing and it relies on the use of Deoxyribonucleoside…

Q: A result of agarose gel electrophoresis of plasmid DNA is shown below. The direction of the…

A: When we isolate the plasmid it can be in relaxed, nicked or supercoiled forms. The supercoiled form…

Q: Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains…

A: Sickle cell anemia is a result of a mutation in the HBB gene. This gene contains the restriction…

Q: 1. Restriction enzymes can occasionally cut at site(s) other than its restriction site. 2. DNA…

A: NOTE:- As you have posted multiple questions, we will solve first two parts for you, To get the…

Q: A colleague of yours is studying a genetic disease and trying to determine its location on a…

A: Gene is the sequence of nucleotide that encodes a particular protein.

Q: The right-hand column contains descriptions of 5 types of molecular markers. Label each description…

A: These markers are pivotal tools within the field of genetics and genomics, helping researchers…

Q: Correctly match the material/supply to its role (job) in genetic engineering. vcuts gene and…

A: Answer 2. The correct match and its role (job) in genetic engineering is 1. Cuts gene and recipient…

Q: The restriction enzyme Xho I recognizes and cuts this sequence: 5-C/TCGAG-3. The restriction enzyme…

A: In molecular biology procedures, restriction enzymes are especially important for DNA cloning. They…

Q: Prepare a simple flowchart of the experimental procedures for Bacteria Preparation and…

A:

Q: 3) Whole-mount in situ hybridization can be used to test for the function of non-coding DNA region.…

A: * Whole mount in situ hybridization is a tool that aids for temporal and spatial dissection of gene…

Q: Using the chart posted to the left of the microwave in lab, what is the 3. restriction recognition…

A: Step 1 In 1963, Arber discovered that Escherichia coli is able to protect itself from the attack of…

Q: Give the interpretation of the given images

A: Gel electrophoresis is a widely used laboratory technique for separating and analyzing DNA, RNA, or…

Q: A Western blot is seen in the picture above. Sodium dodecyl sulfate gel polyacrylamide gel…

A: The western blot, also known as western blotting, is a molecular biology and immunogenetics…

Q: Construct a detail linear restriction map according to the given information in Table 1 below. Table…

A: Total size of DNA= 11 kb EcoRI has one restriction site cutting it into 2 fragments of 2kb and 9kb…

Q: Cause Would result in the following error Unbalanced nucleotide concentrations a) Sequence errors in…

A: The polymerase chain reaction is the method that is used for making copies of DNA, it is developed…

Q: 1). A cis test of two T4 rll mutants yields 1000 plaques on E. coli B and 10 plaques on E. coli K12.…

A: B.Yes, the rule of two should be used here. The rule of two is a statistical method used to ensure…

Q: the differences between THT and SMT PCB fabrication

A:

Q: Based on the images attached, what does the similarity in the sequences tell you about the ermB gene…

A: A cladogram is a branching tree diagram that depicts a phylogeny estimation of the evolutionary…

Q: Which of the following will lead to DNA cutting more frequently? Choose all that apply.

A: The restriction enzymes are enzymes that cut the DNA at specific nucleotide sequences called the…

Q: Decide on two restriction sites that you can use to clone this into pL4440’s MCS. Identify their…

A: The recombinant DNA technology use plasmid to transfer foreign DNA into the host organism in which…

Q: The following picture shows the ethidium bromide-stained bands obtained by gel electrophoresis of…

A: Electrophoresis is a molecular technique that employs the electrical field to assort biomolecules,…

Q: please solve this with step-by-step calculations and explanations.

A: 1:

Q: You are assigned to analyze the genome of an unknown bacterium. Discuss three major steps that must…

A: Genome is the term used to define all the genetic information present in the body of an organism.…

Q: RNA sequencing can detect DNA mutations typically easier to analyze can detect transcripts with very…

A: Microarray technology is used to measure the expression levels of thousands of genes simultaneously,…

Q: Briefly explain this statement ' Prenatal Molecular Testing " please answer at your own easy words.…

A: The period of pregnancy from conception to the start of labor is referred to as the antenatal…

Q: 1. Upon the banana DNA extraction, what do you see in the top portion of the liquid when you look at…

A: DNA (Deoxyribonucleic Acid)  is the genetic material of all living organisms whether plant, animal,…

Q: a. What is the function of the SDS in the lysis buffer? the NaOH? b. What does the potassium…

A: These question are regarding plasmid DNA isolation

Q: Please determine the relative location of the three restriction enzymes. Enzyme Number of fragments…

A: Restriction enzymes are a type of enzyme that can detect and cleave a certain short sequence of…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.