Next, you combine double-digested pNUT and double-digested pPOD and then ligate the pieces together (forming a new, intact plasmid when two fragments combine). Among the possible combinations that could result are four new recombinant plasmids, created when either of the pNUT fragments (A or B) ligates with either of the pPOD fragments (C or D). What will be the size of each new plasmid show below? Indicate the correct size in basepairs. Bam HI A+C Sacl bp The A+C plasmid is expected to be The A+D plasmid is expected to be bp. bp.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Need help, please. Please refer to the images attached. The questions are in the second image attached asking for basepair lengths. The first image is just for information.
Two plasmids pNUT (5000 bp) and pPOD (6500 bp) are cleaved or double digested using two different endonucleases and the resulting fragments are joined together to generate recombinant plasmid. Restriction endonucleases being used here are Bam HI which has restriction site. and Sac I with restriction site .
These two restriction enzymes will produce different kinds of sticky or protruding ends that can easily be joined together using ligase.
Step by step
Solved in 3 steps with 3 images
