a. Summarize in the Table below the predicted fragment lengths for the TAS2R38 Genotype. b. Genotype TT Tt tt Expected band size(s) after digest 311.9 bpd 2395 293.8 bp 22933bp 2.93 sbp & 293.3 bp Based on the above gel electrophoresis results, what are the TAS2R38 genotype in Lanes 1-6? Explain how you can tell.
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
please just do only part 2 question b
![from the information in your Table 1 (Distance Traveled [cm] vs. Length [bp]), construct a properly
labeled "DNA Standard Curve" with formula using Microsoft Excel (see above). From the Microsoft DNA
2, include fragment size (hp) next to the corresponding fragment length (cm) for Lanes 3-6.
Standard Curve Formula, determine the fragment size (bp) of all unknown bands in Lanes 3-6. In a Table
Take-Home Part to Quiz #9:
This part of next week's Quiz #9 must be handed in at the beginning of your in-person lab. This Take-
Home Quiz is worth one out of five points for Quiz #9.
the information (Bands) in Lane 7 "Marker 100bp Ladder" using Microsoft Excel. First, print a hardcopy
From the gel image below, construct a properly labeled "DNA Standard Curve" from
of the Gel Image below. Next, measure in centimeters (cm) with a ruler from the bottom of each well to
the top of each band for all of Lane 7 Marker 100bp Ladder bands. In a Table I, include the distance
traveled in centimeters of each Band (From your Gel Image Lanes 7) next to the fragment length (in bps)
as shown below under the Base Pairs column. From the information in your Table I (Distance Traveled
[cm] vs. Fragment Length [cm]), construct a properly labeled "DNA Standard Curve" with formula using
Microsoft Excel (see below). From the Microsoft DNA Standard Curve Formula, determine the fragment
-6. In a Taple 2, include fragment size (bp) next to the
(cm) for Lanes 1-6.
size (hel
CO
esponding fragmen.c.c
.
Wells
tt nontaster
U D
1
2
TT taster
It taster
U DU U D
3
5
5
6
MARKER
100 bp
ladder
7
Base Pairs Mass (ng)
<- 1.517
45
- 1.200
- 1,000
- 900
800
-
- 700
- 600
-500/517
-
<- 400
-300
200
- 100
35
95
27
24
21
18
97
38
29
25
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