1). A cis test of two T4 rll mutants yields 1000 plaques on E. coli B and 10 plaques on E. coli K12. Assume neither mutant grows on strain K12. The lysate from the initial coinfection was diluted 5x before addition to E. coli B. a. What is the viral titer (plaque forming units per volume plated)? Assume 1mL was plated and use plaques per 1mL as the units. b. Do you need to use the rule of two here? Why or why not? c. What is the distance (m.u.) between the two mutants? d. What can you conclude about the two mutants?

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Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.