8. Yeast genes have cis-acting elements upstream oftheir promoters, similar to enhancers, called upstreamactivating sequences or UASs. Several target genes involved in galactose utilization are regulated by onetype of UAS called UASG, which has four bindingsites for an activator called GAL4. Two target genesregulated by UASG are GAL7 and GAL10. The GAL80 protein is an indirect repressor of GAL7 and GAL10transcription: At UASG, GAL80 binds to GAL4 protein and blocks GAL4’s activation domain. In thepresence of galactose, GAL80 no longer binds GAL4.In which gene(s) (GAL4 and/or GAL80) shouldyou be able to isolate mutations that allow the constitutive expression of the target genes GAL7 andGAL10 in the absence of galactose? In each case,what characteristics of the protein would the mutationdisrupt?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Topic Video
Question

8. Yeast genes have cis-acting elements upstream of
their promoters, similar to enhancers, called upstream
activating sequences or UASs. Several target genes involved in galactose utilization are regulated by one
type of UAS called UASG, which has four binding
sites for an activator called GAL4. Two target genes
regulated by UASG are GAL7 and GAL10. The GAL80 protein is an indirect repressor of GAL7 and GAL10
transcription: At UASG, GAL80 binds to GAL4 protein and blocks GAL4’s activation domain. In the
presence of galactose, GAL80 no longer binds GAL4.
In which gene(s) (GAL4 and/or GAL80) should
you be able to isolate mutations that allow the constitutive expression of the target genes GAL7 and
GAL10 in the absence of galactose? In each case,
what characteristics of the protein would the mutation
disrupt?

Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Gene expression
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education