2A linear fragment of DNA containing the Insulin receptor gene is shownbelow, where boxes represent exons and lines represent introns. Assumetranscription initiates at the leftmost EcoRI site. Sizes in kb are indicatedbelow each segment. Vertical arrows indicate restriction enzyme recognitionsites for Xbal and EcoRI in the Insulin receptor gene. Horizontal arrowsindicate positions of forward and reverse PCR primers. The Horizontal lineindicates sequences in probe A.Probe AEcoRI Xbalt +XbaI+0.5kb | 0.5 kb | 0.5 kb | 0.5kb| 0.5 kb | 0.5 kb |1.0 kbEcoRIOn the gel below, indicate the patterns of bands expected for each DNA sampleLane 1: EcoRI digest of the insulin receptor geneLane 2: EcoRI + Xbal digest of the insulin receptor geneLane 3: Southern blot of the EcoRI + Xbal digest insulin receptor gene probed withprobe ALane 4: PCR of the insulin receptor cDNA using the primers indicatedMarkers65410.51234

Based on the experiments and expected gel band patterns for each lane, we can draw the following…

Answered: 2 A linear fragment of DNA containing…

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...

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Refer to the succeeding diagram of two DNA samples (A, B) with the specific restriction enzyme (RE) recognition sites marked with arrows. A spontaneous mutation (insertion) occurred in DNA sample A and introduced an additional restriction site. RE site RE site RE site RE site ↓↓ ↓ A RE site RE site RE site ↓ B Design two (2) DNA probes to be used in RFLP analysis involving these two DNA samples. Draw/illustrate the region(s) in the DNA molecule which you will use as reference (homologous) sequences in the design of the probes, THAT - a. Will reveal RFLP polymorphism between the DNA samples after probe detection/visualization; and b. Will not reveal RFLP polymorphism between the DNA samples c. Illustrate and briefly explain the expected result from this RFLP marker analysis. (Assume that the DNA probe is labelled with 32P radioactive isotope).) [answer in maximum 5 sentences ONLY]
The restricition EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3' the restriction enzyme Hindll cleaves at the sequence 5'-AAGCTT-3'. A 20 kb circular plasmid containing OFP gene is digested with each enzyme individually and then in combination, and the resulting fragment sizes are determined using electrophoresis Restriction Enzyme(s) ECORI alone Fragment length 6 kb ,14 kb 7 kb , 13 kb 2kb, 4kb, 5 kb, 9kb Hindlll alone EcoRI and Hindll Construct the circular molecule and indicate the relative positions of the EcoRI and Hindlll restriction sites
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Relative to this image of the steps in gene cloning, identify the steps involved and the protein/enzyme requirements of each.
CRISPR-cas9 is a powerful technique for gene editing. Please place the stages of a CRISPR-cas9 gene editing workflow in the correct order below options are on the right
A cloned Bam HI DNA fragment, 3.0 kb long, was isolated in preparation for sequence determination. First, a map of restriction endonuclease cleavage sites had to be prepared. Cleavage by each of the indicated enzymes generated fragments of the sizes shown on the three agarose electrophoresis gel figures below; the gels are running from top to bottom. On the diagram below those, show the relative positions of EcoR I and Hpa II cleavage sites and the distances (in kb) between them. Explain your reasoning – is your placement of the cleavage sites the only one that will work?
Below is a plasmid with restriction sites for Baml and EcoRI, Several restriction digests were done using these two enzymes either alone or in combination. Hint: Begin by determining the number and size of the fragments produced with each enzyme. "kb" stands for kilobases, or thousands of base pairs. Plasmid Gel lanes I | III IV V 6 Kb Bam HI Bam HI. 20 Kb PGEN101 (20 Kb) 2 Kb 11 Kb Bam HI 8 Kb 8 Kb 4 Kb 6 Kb Eco RI 3 Kb 21. Which lane shows a digest with BamHl only? a. I b.I c. II d. IV e. V 22. Which lane shows a digest with EcoRLonly? a. I 23. Which lane shows the fragments produced when the plasmid was incubated with both EcoRI and BamH1? a. I b.I c. II d. IV e. V b. I c. II d. IV e. V Base pairs
What is the principle of the Restriction Fragment Length Polymorphism (RFLP) in the diagnosis of human diseases? O a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one OC. The size of a band digested by specific restriction enzymes is different from the expected one O d. The DNA band detected by Southern blot is different from that by Northern blot
1. Briefly explain why the total size of the pMBBS plasmid in the Restriction Enzymes practical is 3000bp (base pairs) 2. Briefly explain why the cut sites on the pMBBS plasmid in each Restriction Enzymes (EcoRI, BamHI, and XhoI) just 1 each 3. Briefly explain what led to the 5 fragments formed which linked to the 500bp, 1000bp, 1500bp, 2000bp, 2500bp sizes
1. Explain how the SNP changes the recognition sequence for the restriction enzyme and affects its ability to cut the DNA. Indicate which of the 2 alleles (taster or non-taster) is cut by the restriction enzyme. 2. Predict the size of the expected DNA fragments after restriction digest for the two alternative SNP sequences (the taster and the non-taster).i.) Taster allele after being cut ii.) Non-taster allele after being cut
Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant sickle cell anemia allele of beta globin. This mutation destroys an MstII restriction site normally present in the beta globin gene. This difference between the normal allele and the mutant allele can be detected with Southern blotting. Using a labeled beta globin gene as a probe, what differences would you expect to see for a Southern blot of the normal beta globin gene and the mutant sickle cell gene?

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