Explain the predicted results with the diagrams presented. The cut plasmid is 5.9 kb, and a gene of interest is 720 bp. Circle = Ncol cut; triangle = Not! cut 1) No ligation 2) Self-ligation of two vectors 3) Self-ligation of two inserts 4) Ligation of a vector with multiple inserts. Also, how many inserts can be ligated into a vector? 5) Ligation of one insert into a vector

Explain the predicted results with the diagrams presented. The cut plasmid is 5.9 kb, and a gene of interest is 720 bp. Circle = Ncol cut; triangle = Not! cut 1) No ligation 2) Self-ligation of two vectors 3) Self-ligation of two inserts 4) Ligation of a vector with multiple inserts. Also, how many inserts can be ligated into a vector? 5) Ligation of one insert into a vector

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter9: Molecular Biology

Section: Chapter Questions

Problem 15CTQ: Transcribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’

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