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Explain the predicted results with the diagrams presented. The cut plasmid is 5.9 kb, and a gene of interest is 720 bp. Circle = Ncol cut; triangle = Not! cut 1) No ligation 2) Self-ligation of two vectors 3) Self-ligation of two inserts 4) Ligation of a vector with multiple inserts. Also, how many inserts can be ligated into a vector? 5) Ligation of one insert into a vector

Explain the predicted results with the diagrams presented. The cut plasmid is 5.9 kb, and a gene of interest is 720 bp. Circle = Ncol cut; triangle = Not! cut 1) No ligation 2) Self-ligation of two vectors 3) Self-ligation of two inserts 4) Ligation of a vector with multiple inserts. Also, how many inserts can be ligated into a vector? 5) Ligation of one insert into a vector

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Explain the predicted results with the diagrams presented. the cut plsmid is 5.9kb, and a gene insert is 720 bp.

1) ligation

2) self ligation of two vectors

3) self ligation of two inserts

4) ligation of a vector with multiple inserts. also how many inserts can be ligated into a vector

5) ligation of one insert into a vector 

Explain the predicted results with the diagrams presented. The cut plasmid is 5.9 kb, and a gene of interest is 720 bp. Circle = Ncol cut; triangle = Not! cut 1) No ligation 2) Self-ligation of two vectors 3) Self-ligation of two inserts 4) Ligation of a vector with multiple inserts. Also, how many inserts can be ligated into a vector? 5) Ligation of one insert into a vector
Transcribed Image Text:Explain the predicted results with the diagrams presented. The cut plasmid is 5.9 kb, and a gene of interest is 720 bp. Circle = Ncol cut; triangle = Not! cut 1) No ligation 2) Self-ligation of two vectors 3) Self-ligation of two inserts 4) Ligation of a vector with multiple inserts. Also, how many inserts can be ligated into a vector? 5) Ligation of one insert into a vector
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