Colour of band Plant pigment Table 1. Chromatographic analysis of green leaf Distance moved by component Distance moved by Rf value (cm) solvent (cm) Light brown Chlorophyll B 1.5 8.9 0.17 Yellowish green Dark brown Chlorophyll B tannin 6.5 8.9 0.73 7.1 8.9 0.80 Table 2. Chromatographic analysis of the variegated leaf sample Colour of band Plant pigment Distance moved by component Distance moved by Rf value (cm) solvent (cm) Yellowish green Chlorophyll B 2.5 10.2 0.25 Light orange Carotene 5.5 10.2 0.54 Light purple Anthocyanin 7.2 10.2 0.71 Brown Tannin 7.5 10.3 0.74 Table 3. Chromatographic analysis for hibiscus sample Colour of band Plant pigment Distance moved by Distance Rf value component moved by (cm) solvent (cm) Pale yellow Pink Purple | Xanthophyll 1 9.5 0.1 Anthocyanin 5.5 9.5 0.58 tannin 6.9 9.5 0.73

Colour of band Plant pigment Table 1. Chromatographic analysis of green leaf Distance moved by component Distance moved by Rf value (cm) solvent (cm) Light brown Chlorophyll B 1.5 8.9 0.17 Yellowish green Dark brown Chlorophyll B tannin 6.5 8.9 0.73 7.1 8.9 0.80 Table 2. Chromatographic analysis of the variegated leaf sample Colour of band Plant pigment Distance moved by component Distance moved by Rf value (cm) solvent (cm) Yellowish green Chlorophyll B 2.5 10.2 0.25 Light orange Carotene 5.5 10.2 0.54 Light purple Anthocyanin 7.2 10.2 0.71 Brown Tannin 7.5 10.3 0.74 Table 3. Chromatographic analysis for hibiscus sample Colour of band Plant pigment Distance moved by Distance Rf value component moved by (cm) solvent (cm) Pale yellow Pink Purple | Xanthophyll 1 9.5 0.1 Anthocyanin 5.5 9.5 0.58 tannin 6.9 9.5 0.73

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter6: Biomedical Electrodes, Sensors, And Transducers

Section: Chapter Questions

Problem 10Q

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Pigment Spot Color Distance Passed Partition (mm) Coefficient, R Chlorophyll b Yellow green 22 22/54 0.407 Chlorophyll a Blue green 25 25/54 = 0.462 Xanthophyll Bright yellow Yellow orange 30 30/54 = 0.555 Carotene 52 52/54 = 0.962 Distance passed by the solvent system (solvent front) 54mm a. Which pigment travelled the longest distance? What does this tell you about that pigment? b. Which pigment travelled the shortest distance? What does that tell you about that pigment? h (US.) DELL ... end F12 insert home prt sc F10 DII F3 F5 F6 F7 F9 F11 F4 2$ 4. 7 8. 9. Y U F G K L D ...- エ < LO LU
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